Author: Eagle Biosciences

The Eagle Bioscience’s Dopamine ELISA Assay Kit was utilized in a recent publications! The aim of the study was to investigate the potential of iron oxide nanoparticles produced using ascorbic acid (AA-IONPs) against Parkinson’s Disease. Check out the abstract and full text below!

Abstract

One of the most prevalent neurological movement diseases affecting the geriatric population globally is Parkinson’s disease (PD). Recent studies have highlighted the potency of biomolecules in the generation of nanomaterials and also over their impact on neuroprotection. The objective of this research was to investigate the potential of iron oxide nanoparticles produced using ascorbic acid (AA-IONPs) against PD. Numerous analytical methods including UV–Vis analysis, Fourier-Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and electron microscopy (SEM, TEM), were used to analyze the produced AA-IONPs. Nitric oxide, prostaglandin E2, and inflammatory cytokines analyses such as IL-6 and IL-1 were employed to assess the neuroprotective effect of synthesized AA-IONPs on inflammatory agent lipopolysaccharides driven murine microglial BV2 cells. And also Parkinson-induced C57BL/6 mice were given the nanoparticle treatment to confirm the in vivo effects of the produced nanoparticles. Our characterization findings had demonstrated that AA-IONPs have a significant role in acting as an ideal nano drug and may have the ability to reduce inflammation in in vitro murine microglial BV2. The outcomes of in vivo tests conclusively show that AA-IONPs had reduced neuroinflammation and enhanced motor coordination in Parkinson’s disease-induced rats.

Li, L., Luo, P., Wu, S. et al. Deciphering the neuroprotective effect of ascorbic acid mediated synthesis of iron oxide nanoparticles against Parkinson’s disease: an in vitro and in vivo approach. Macromol. Res. 31, 949–960 (2023). https://doi.org/10.1007/s13233-023-00186-x

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The Biomedica Human IL-6 High Sensitive ELISA Assay Kit was highlighted in a recent publication that explored factors associated with incident vertebral fractures in glucocorticoid-treated Duchenne muscular dystrophy. Check out the abstract and full text below!

Abstract

Purpose: Prevention of fractures is an unmet need in glucocorticoid (GC)-treated Duchenne muscular dystrophy. This study explored factors associated with incident vertebral fractures (VFs) to inform future fracture prevention efforts.

Methods: VFs were evaluated prospectively at study baseline and 12 months on lateral spine radiographs in participants aged 4 to 25 years with Duchenne muscular dystrophy. Clinical factors were analyzed for their association with the change in Spinal Deformity Index (sum of the Genant-defined VF grades from T4 to L4) between baseline and 12 months.

Results: Thirty-eight males were evaluated (mean ± SD age at baseline 11.0 ± 3.6 years; mean ± SD GC duration at baseline 4.1 ± 3.1 years; 74% ambulatory). Nine of 38 participants (24%) had 17 incident VFs, of which 3/17 VFs (18%) were moderate/severe. Participants with 12-month incident VF had lower mean ± SD baseline lumbar spine areal bone mineral density Z-scores (-2.9 ± 1.0 vs -1.9 ± 1.1; P = .049) and lower total body less head areal bone mineral density Z-scores (-3.1 ± 1.2 vs -1.6 ± 1.7; P = .036). Multivariable linear regression showed that at least 1 VF at baseline (P < .001), a higher number of antecedent non-VF (P < .001), and greater bone age delay at baseline (P = .027) were significant predictors of an increase in the Spinal Deformity Index from baseline to 12 months.

Conclusion: The observation that ≥ 1 prevalent VF and/or non-VF were the strongest predictors of incident VFs at 12 months supports the need for prevention of first fractures in this high-risk setting. Bone age delay, a marker of GC exposure, may assist in the prioritization of patients in efforts to prevent first fractures.

Keywords: Duchenne muscular dystrophy; bone fragility; glucocorticoids; incident fractures; osteoporosis; vertebral fractures.

Risk Factors Associated with Incident Vertebral Fractures in Steroid-treated Males with Duchenne Muscular Dystrophy . Phung K, McAdam L, Ma J, McMillan HJ, Jackowski S, Scharke M, Matzinger MA, Shenouda N, Koujok K, Jaremko JL, Wilson N, Walker S, Hartigan C, Khan N, Page M, Robinson ME, Saleh DS, Smit K, Rauch F, Siminoski K, Ward LMJ Clin Endocrinol Metab. 2023 Aug 23:dgad435. doi: 10.1210/clinem/dgad435. Epub ahead of print.PMID: 37610420

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The Eagle Bioscience’s 25-OH Vitamin D ELISA Assay Kit has been used in a number of recent publications! These studies range from exploring the effects of vitamin D on cellular responses, molecular immunity, and mycobacterial killing in cattle, to the role of vitamin D, DKK1, hepcidin, and other oxidative stress biomarkers in type 2 diabetes mellitus patients. Check them all out below!

Flores Villalva, Susana. “The Effects of Vitamin D on the Cellular Responses, Molecular Immunity, and Mycobacterial Killing in Cattle.” University College Dublin. School of Agriculture and Food Science, 2022.

Kamel, Amira A., et al. “The Role of Vitamin D, DKK1, Hepcidin and Oxidative Stress Biomarkers in Type 2 Diabetes Mellitus Patients with and without Diabetic Nephropathy.” The Egyptian Journal of Hospital Medicine, vol. 89, no. 2, 2022, pp. 7137–7146.

Blakely, L.P., et al. “Effect of Vitamin D Source and Amount on Vitamin D Status and Response to Endotoxin Challenge.” Journal of Dairy Science, vol. 106, no. 2, 2023, pp. 912–926.

Fernández-Lázaro, Diego, et al. “25-Hydroxyvitamin D Serum Levels Linked to Single Nucleotide Polymorphisms (Snps) (RS2228570, RS2282679, rs10741657) in Skeletal Muscle Aging in Institutionalized Elderly Men Not Supplemented with Vitamin D.” International Journal of Molecular Sciences, vol. 23, no. 19, 2022, p. 11846.

Kumar, Abhai, et al. “Vitamin D and Inflammatory Cytokines Association in Mild Cognitive Impaired Subjects.” Neuroscience Letters, vol. 795, 2023, p. 137044.

Stenhouse, Claire, et al. “Uptake of Phosphate, Calcium, and Vitamin D by the Pregnant Uterus of Sheep in Late Gestation: Regulation by Chorionic Somatomammotropin Hormone.” International Journal of Molecular Sciences, vol. 23, no. 14, 2022, p. 7795.

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