Dopamine ELISA Assay Kit

$520.00

The Eagle Biosciences Dopamine ELISA Assay Kit is intended for the quantitative determination of Dopamine in urine or plasma. The Dopamine ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

Dopamine ELISA Assay Kit

For Research Use Only

Size:  1×96 wells
Sensitivity:  Urine:  0.44 ng/ml, Plasma:  29 pg/ml
Dynamic Range:  1.5 – 2560 ng/ml (9.8 – 16717 nmol/L)
Incubation Time:  Overnight
Sample Type:  Urine, Plasma
Sample Size: 15 µL (Urine), 300 µl (Plasma)

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika


Reference Values (Normal)

  • Urine < 600 µg/day
  • Plasma  < 100 pg/ml
    *It is suggested that each laboratory establish its own normal ranges

Additional Information

Assay Background

The Eagle Biosciences Dopamine ELISA Assay Kit is intended for the quantitative determination of Dopamine in urine or plasma. The Dopamine ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.  Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis. The Dopamine ELISA assay kit provides materials for the quantitative measurement of dopamine in plasma and urine. Dopamine is extracted using a cis-diol-specific affinity gel and acylated to N-acyl-dopamine and then converted enzymatically into N-acyl-3-methoxytyramine.

Assay Principle

The competitive Dopamine ELISA Assay Kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated dopamine from the sample and solid phase bound dopamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase dopamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase dopamine is inversely proportional to the dopamine concentration of the sample.

Typical Standard Curve

Manual

Product Manual


Publications

Citations

Yadav, Gaya, et al. “Novel Organelle Anion Channels Formed by Chromogranin B Drive Normal Granule Maturation in Endocrine Cells.” BioRxiv, 17 Apr. 2018, doi:10.1101/302828.Kandil, EA;Abdelkader, NF;El-Sayeh, BM;Saleh, S;, (2016). Imipramine and amitriptyline ameliorate the rotenone model of Parkinson’s disease in rats, 33226-37.

Abu Ghazaleh, H;Lalies, MD;Nutt, DJ;Hudson, AL; The modulatory action of harmane on serotonergic neurotransmission in rat brain., Brain Res.;2015, p57-64, Vol. 1597. DOI: 10.1016/j.brainres.2014.11.056

Simpson, N;Maffei, A;Freeby, M;Burroughs, S;Freyberg, Z;Javitch, J;Leibel, RL;Harris, PE, Dopamine-mediated autocrine inhibitory circuit regulating human insulin secretion in vitro, Mol. Endocrinol., 2012, V. 26, Page, 1757-72,