The ELISpot Assay Kits utilize sandwich immuno-enzyme technology to measure the number of cells secreting the cytokine of interest in a sample. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates. The end result is visible spots on the on the surface of the plate. Each spot corresponds to an individual cell that was secreting the target protein.
The information provided by ELISpot Assay Kits is applicable to various fields of study including: transplantation, vaccine development, T-cell regulation analysis, autoimmune disease, cancer, allergy, and viral infections. These kits are highly sensitive and easy to use.