GA-map Technical Spotlight

This week Eagle Biosciences is highlighting a revolutionary test in the microbiome industry. The GA-map® Dysbiosis Test Lx was developed by Genetic Analysis in Norway. The GA-map ® is a tool that allows mapping of the intestinal microbiota profile for a selected set of bacteria in a sample. The test is based on advances in DNA profiling using probes targeting variable regions (V3 to V7) of the bacterial 16S rRNA gene to characterize and identify bacteria present. The targets are identified in a molecular multiplex assay that utilizes the Single Nucleotide Primer Extension (SNuPE) technology patented by professor Knut Rudi of the Norwegian University of Life Sciences (​NMBU) (US6617138). A unique algorithm takes advantage of all the data generated by the detection of the SnuPE products to determine the microbiota profile in the sample. The algorithm is incorporated in the GA-map® Dysbiosis Analyzer software that accompanies the test.

Check out this video for a quick overview of the GA-map ® Dysbiosis Test Lx Platform equipment and techniques.

Note: This test is for research use only and not to be used for diagnostic procedures

If you have any questions about the GA-map® Dysbiosis Test Lx or our other offerings contact us here.

Polystreptavidin R (Polystrept R) reagent is for the surface coating of plastics, membranes, beads and etc. with maximum biotin binding capacity. It improves the signal-noise-relation in testing and can help save on material costs.

About Polystreptavidin R (Polystrept R)

Polystrept R is a chemically modified polymerized Streptavidin characterized by an extraordinary high Biotin binding capacity. Polystrept R coatings on solid phases offer a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in a biotinylated form. Coatings made of Polystreptavidin R combine the excellent binding capacity with a high chemo and thermo stability and a long shelf life. It is suitable for coatings of membranes, beads, biochips, plastics etc.

Learn about the benefits of using Polystrept R in your research in this video from Biotez!

Why use streptavidin-coated microplates and Polystrept R with lateral flow test strips??

  • Convenient method for a uniform and stable immobilization of biotinylated molecules
  • Immobilization of Biomolecules on solid phases in a directed manner, while avoiding or minimizing denaturation
  • Ready-to-use, no washing or blocking steps required
  • High signal-to-noise ratio
  • Low nonspecific binding
  • Long shelf life
  • Lower reagent consumption and especially enormous cost savings with lateral flow test stripes

Related Posts

If you have any questions about Polystreptavidin R or our other offerings, contact us here.

ELISA for Detecting SARS-CoV-2 Mutant Strains

SARS-CoV-2, a single stranded RNA virus, has quickly become the subject of much research worldwide. Coronaviruses are composed of four primary proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid (N). The spike protein (S-protein) performs two primary tasks that aid in host infection: 1) mediates the attachment between the virus and host cell surface receptors such as ACE2, and 2) facilitates viral entry into the host cell by assisting in the fusion of the viral and host cell membranes. In December of 2020 three new SARS-CoV-2 variants came to light, UK B.1.1.7, South Africa B.1.351 and Brazil P.1/P.2.

Eagle Biosciences offers a SARS CoV-2 Spike Protein Antigen Quantitative ELISA Assay Kit that is highly sensitive. Clinical studies show that this kit is effective at detecting the spike protein antigen in all three major circulating SARS-CoV-2 variant strains. Click here to learn more.

About the SARS-CoV-2 Spike Protein Antigen Quantitative ELISA Assay Kit

  • Validated for both serum and plasma samples.
  • No cross-reactivity with other respiratory pathogens.
  • Standardization and reproducibility, with lot-to-lot consistency.

Eagle Biosciences is dedicated to providing support to researchers in their studies of SAR-CoV-2 and the new variants. To hear more about our COVID-19 resources and other offerings, contact us here.

iLite Assay Ready Cells

iLite® Assay Ready Cells are developed by our partners at Svar Life Science. iLite technology is based upon a reporter gene assay format, modified and adapted for applications during the whole drug development cycle as well as for monitoring of biological drugs. These cell lines can be developed for any biopharmaceutical target and assays for drug potency, i.e. drug activity, and neutralizing antibodies (NAbs) can easily be set-up using the same cell line. The Assay Ready cells are genetically engineered to be used with a reporter gene assay technique for detection the the drug potency and the NAbs.

A Message from SVAR about iLite:

“Many therapeutic antibodies that are used to treat common disorders, such as cancer and autoimmune diseases work – at least in part – by activating ADCC.

Our reporter gene-based iLite® ADCC bioassays offer a convenient and powerful way of measuring the ability of an antibody to elicit ADCC.

Read our new white paper and learn about the performance of our ADCC bioassays and how they compare to a leading competitor in terms of sensitivity and dynamic range.”

Download the White Paper here.

Find all of the iLite products offered on the iLite Assay Ready Cells product page.

ELISpot Kits

Eagle Biosciences proudly offers a broad range of products in our extensive catalog. In addition to the well known ELISA format, we offer several assay kits in a enzyme-linked immunospot (ELISpot) assay format.


ELISAs use antibodies to detect the presence and concentration of a protein in a liquid sample. In the most common form, antigens are applied to a stable surface (usually a microtiter plate). Then the corresponding antibody, linked with an enzyme, is added to form a complex. Finally, the enzyme’s substrate is added, which produces a measurable reaction, usually in the form of a color change.

ELISpots also utilize antibody-enzyme reactions. However, instead of measuring the concentration of protein in a sample, they measure the number of cells secreting the cytokine of interest in a sample. Antigens are applied to the plate surface, and when samples are added, cells settle on the bottom of the wells. The cytokines secreted by the cells are captured by the surrounding antigens. After the appropriate incubation time, the cells are removed and the secreted cytokine is detected using a detection antibody linked with an enzyme. The enzyme’s substrate is added, producing a reaction. The end result is visible spots on the on the surface of the plate. Each spot corresponds to an individual cell that was secreting the target protein.

The information provided by ELISpot kits is applicable to various fields of study including: transplantation, vaccine development, T-cell regulation analysis, autoimmune disease, cancer, allergy, and viral infections. These kits are highly sensitive and easy to use.

ELISpot Kit Procedure

Our available kits:

IFN gamma ELISpot Assay Kit

Mouse IFN gamma ELISpot Assay Kit

Rat IFN gamma ELISpot Assay Kit

TNF alpha ELISpot Assay Kit

Rat TNF alpha ELISpot Assay Kit

Contact us if you are looking for a specific ELISpot or if you have questions about any of our products.

A new article published by Nelly Kanberg, et al. titled Neurochemical evidence of astrocytic and neuronal injury commonly found in COVID-19 has set out to study how COVID-19 impacts the central nervous system. It’s already known how this novel coronavirus effects the respiratory system, but does the body’s inflammatory response to this virus cause lasting effects to the nervous system?

For this study, two biomarkers in human plasma were measured; neurofilament light chain protein (NfL) and glial fibrillary acidic protein (GFAP). The samples came from patients who had mild, moderate or severe cases of COVID-19 (n=47). Of those 47 patients, the ones who had a severe case of COVID-19 showed higher concentrations of GFAP and NfL.

Glial Fibrillary Acidic Protien, or GFAP for short, is a known biomarker in the central nervous system (CNS) that is typically studied in those with brain injuries. When a brain injury occurs (concussion, retinal stess, tumors, etc.), GFAP is released into the blood stream and helps determine the severity of the injury. The results of this study help support and determine the relationship between COVID-19 and potentially lasting neural injuries.

To read this article in full, click here.

To learn more about how to measure GFAP, click here or contact us with your questions

Eagle Biosciences proudly offers a broad range of products in our extensive catalog. In addition to the well known ELISA format, we offer several assay kits in a Chemiluminescent Enzyme Immunoassay (CLEIA) format.


ELISAs use antibodies to detect the presence of a protein in a liquid sample. In the most common form, antigens are applied to a stable surface (usually a microtiter plate). Then the corresponding antibody, linked with an enzyme, is added to form a complex. Finally, the enzyme’s substrate is added, which produces a measurable reaction, usually in the form of a color change.

CLEIAs also utilize antibody-enzyme reactions, but offer a higher level of sensitivity due use of chemiluminescence rather than colorimetric absorbance or fluorescence. Chemiluminescence allows detection of trace amounts of analytes because samples typically have little to no background interference. These reactions are also easily measured using a photomultiplier or photodiode. These types of kits are increasing in popularity due to their increased sensitivity, specificity, reliability, and reproducibility.

Our available CLEIA/CLIA kits

GFAP (Glial Fibrillary Acidic Protein) CLIA Assay Kit
MedFrontier FGF23 (Human Intact FGF23) CLEIA ELISA Assay

Contact us if you are looking for a specific CLEIA or if you have questions about any of our products.

DotDiver2.0 is a fully automatic processor for Dot/LINE immunoassays manufactured by German biotech Generic Assays.

DotDiver2.0 processes and evaluates assay kits with minimal user intervention. It can perform up to 24 tests at one time. It features bar code identification for test strips and cartridges. DotDiver provides digitized results, and it is LIMS ready or can be set up to print on paper. All you have to do is load the tests and press start, no need to monitor while it’s running. The system requires minimal maintenance and takes up little space.

DotDiver2.0 assays are available for:

  • Celiac disease / Crohn‘s disease
  • Pernicious anemia
  • Neuropathies
  • Anti-phospholipid syndrome
  • Systemic lupus erythematosus
  • Vasculitis
  • Nephritis
  • Myositis
  • Scleroderma
  • Autoimmune hepatitis

For more information, see the DotDiver 2.0 Immunoassay Processor product page.

Contact us for more information about the DotDiver2.0 or Dot assays.

What are MHC molecules?

Major histocompatibility complex (MHC) molecules play an important role in the acquired immune system of vertebrates. MHC molecules present peptides derived from pathogens on the cell surface so that T-cells can determine the appropriate immune response. The MHC also plays a role in mediating leukocyte interactions, determining compatibility for organ transplants, and determining autoimmune disease susceptibility. In humans, the MHC complex is also known as the human leukocyte antigen (HLA) complex.

The peptide-MHC (pMHC) interaction to cognate T-cell receptors (TcR) occurs rapidly and at low affinity. Tetramerizing these molecules on a streptavidin scaffold engages multiple TcRs expressed on a given T cell, which stabilizes the reaction and allows for specific T cell staining. pMHC monomers and tetramers can also be used for purification and manipulation of T cells.

Research Applications

MHC monomers and tetramers can be used for selection and proliferation of specific T cells, allowing researchers to isolate specific viral or tumor related antigens. These antigens can be reintroduced to augment the immune system. They are also used in organ transplant research to help reduce the risk of graft-versus-host disease. Additionally, researchers in cancer immunotherapy and vaccine development are exploring various MHC multimer applications to further their fields.

What Eagle Biosciences Offers

We offer a wide range of pMHC monomers and tetramers through our partner, ImmunAware, including easYmer MHC tetramer kits. All of the MHC molecules in are catalog are biotinylated, meaning all of the pMHC monomers can be tetramerized with the laboratory’s choice of strepatavidin label.

View all of our monomer, tetramer, and easYmer kits here.

For more information or assistance finding a specific product, please contact us.

What is Metal Enhanced Fluorescence?

Metal Enhanced Fluorescence (MEF) is based on the reaction of excitation light with electrons of metal nano-structures to generate strong electromagnetic fields, also known as LSPs (localized surfaced plasmons). When LSPs are bound to the surface of the metal nano-structure, it creates a plasmonic structure, which can enhance the signal of the phosphores more than 100 times.

About our FluoBolt™ MEF Assays

Eagle Biosciences proudly offers a line of Metal Enhanced Fluorescence Immunoassays from our partner, Fianostics. These products from the FluoBolt™ line are highly sensitive, single-step assays that require no enzyme substrates. FluoBolt™ MEFs are completely compatible with the 96-well ELISA format and provide a stable signal over time.

We currently offer the following FluoBolt™ MEF Immunoassays:

With many more to come!

Contact us for more information about FluoBolt™ products.