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A new article published by Nelly Kanberg, et al. titled Neurochemical evidence of astrocytic and neuronal injury commonly found in COVID-19 has set out to study how COVID-19 impacts the central nervous system. It’s already known how this novel coronavirus effects the respiratory system, but does the body’s inflammatory response to this virus cause lasting effects to the nervous system?

For this study, two biomarkers in human plasma were measured; neurofilament light chain protein (NfL) and glial fibrillary acidic protein (GFAP). The samples came from patients who had mild, moderate or severe cases of COVID-19 (n=47). Of those 47 patients, the ones who had a severe case of COVID-19 showed higher concentrations of GFAP and NfL.

Glial Fibrillary Acidic Protien, or GFAP for short, is a known biomarker in the central nervous system (CNS) that is typically studied in those with brain injuries. When a brain injury occurs (concussion, retinal stess, tumors, etc.), GFAP is released into the blood stream and helps determine the severity of the injury. The results of this study help support and determine the relationship between COVID-19 and potentially lasting neural injuries.

To read this article in full, click here.

To learn more about how to measure GFAP, click here or contact us with your questions

Svar Life Sciences has Added Two New assay ready cells to their iLite® Product Line!


iLite® C5a Assay Ready Cells

The iLite® C5a Assay Ready Cells can be used for the quantification of C5a activity, and for determination of inhibitory activity against either C5a itself or against the C5a receptor in test samples, including human serum.

Read Product Specifications Here


iLite® RANKL Assay Ready Cells

The iLite® RANKL Assay Ready Cells can be used for the quantification of RANKL activity, RANKL inhibitor activity or immunogenicity studies and determination of neutralizing antibody response against RANKL inhibitors in test samples, including human serum.

Read Product Specifications Here

 

As always, contact us with any questions you have and someone from our sales team will be happy to assist you!

Comparison of the Elecsys® Anti-SARS-CoV-2 immunoassay with the EDITM enzyme linked immunosorbent assays for the detection of SARS-CoV-2 antibodies in human plasma

Both of Epitope Diagnostics’ Anti-SARS-COV-2 antibody assays (IgG and IgM) have been featured in a head-to-head comparison against Elecsys® Anti-SARS-CoV-2. Elecsys® Anti-SARS-CoV-2 is manufactured by Roche Diagnostics and has been the gold-standard in the diagnostics industry for automated assays.

Abstract: Recently, Roche Diagnostics (Rotkreuz, Switzerland) has launched the IVD CE-marked Elecsys® Anti-SARS-CoV-2 assay for the qualitative detection of SARS-CoV-2 antibodies on the cobas e immunoassay analyzers. The aim of this study was to compare the clinical performance of the Elecsys® Anti-SARS-CoV 2 assay with the EDITM SARS-CoV-2 IgM and IgG enzyme linked immunosorbent assays (ELISA), which we have recently established in our laboratory.

Read and Learn More Here


Coronavirus COVID-19 IgG ELISA Assay

Coronavirus COVID-19 IgM ELISA Assay Kit

We, at Eagle Biosciences, Inc. are rapidly expanding our selection of Coronavirus Assays by welcoming two new research kits! With the ongoing pandemic, it’s more important now than ever that we continue to offer the highest quality assays to our customers. Here are our newest additions;


Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay
Catalog Number: E111

The Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay Kit is manufactured in Germany by Mediagnost. This assay is a highly specific enzyme immunoassay for the detection of IgG antibodies directed against SARS-CoV-2-S1 Receptor Binding Domain (RBD) in human blood. In this assay the recombinant Receptor Binding Domain (RBD) of SARS-CoV-2 S1 spike protein, which binds the ACE2 receptor, is used. The use of RBD increases the specificity of the assay since the domain is identical with SARS-CoV but not with MERS-CoV for example. Antibodies directed against the RBD neutralize both virus strains SARS-CoV and SARS-CoV-2.

Size: 1×96 wells
Incubation Time: 2 hours 40 minutes
Sample Type: Serum and Plasma
Sample Size: 100 µl
Controls Included

To learn more about the Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay click here*


Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit
Catalog Number: 3940

The Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is manufactured in Germany by Generic Assays. This assay is a module based Enzyme Immunoassay for the confirmation of positive IgG antibodies against SARS coronavirus 2 (SARS-CoV-2) in the first screening. The Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit determines the specificity of antibodies against the main immunodominant antigens (Spike Glycoprotein 1, Spike Glycoprotein 2, Nucleocapsid) of SARS-CoV-2 in human serum or plasma. This test kit consists of modules separately coated with the major antigens of the virus as seen in the illistration below:

Size: 96 wells (24 samples x 4)
Incubation Time: 2 hours
Sample Type: Serum or Plasma
Number Of Tests Per Kit: 24 (22 samples + controls)
Sample Size: 50 µl
Controls Included

To learn more about the Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit click here*

 

Check out our entire Coronavirus Series here or contact us with any questions or inquires

 

*These kits are for research use only and should not be used for diagnostic procedures.

Eagle News

Immune Monitoring COVID-19

Cells infected with SARS-CoV-2 are only visible to CD8+ T cell after virus proteins have been processed and presented by MHC class I molecules – and only then are the CD8+ T cell able to eradicate the infection. While antibodies against the COVID-19 virus are important to prevent or minimize infection, CD8+ T cells are responsible for clearing the virus from the body. Hence, the identification of peptides being presented by MHC class I is essential to:

  • Design potent vaccines eliciting a persistent response and
  • Using MHC class I tetramers to monitor cellular immune responses
  • Assessments of vaccine induced cellular immunity

immunAware’s MHC class I easYmers allow researchers pursuing novel COVID-19 vaccines to generate tetramers and monitor immune responses. In the current pandemic scenario it is essential not to only focus on a few ALA allotypes; rather it is essential to be able to monitor as many allotypes as possible to ensure a novel vaccine targets a broad range of allotypes. Our portfolio of easYmers cover 34 HLA allotypes (HLA-A,-B,-C) which is further supplemented by HLA molecules available for custom tetramer production, bringing the number of available allotypes up to 81. Thus, our range of allotypes ensures a coverage to a 2% frequency in the caucasian population.

The easYmer reagents can also be used to validate the binding of predicted epitopes to further stratify the selection of potential COVID-19 vaccine targets.

As the US distributor for immunAware, Eagle Biosciences offer their extensive catalog of easYmer and MHC tetramer products, including:
easYmer HLA-C*07:02 MHC Tetramers Kit
HLA-A*29:02 (WYMWLGARY) Class I Tetramer
easYmer HLA-B*15:01 MHC Tetramers Kit

See our full list of immunAware products here. If you have questions or for assistance with a specific product, please contact us.

Eagle Biosciences, Inc. is excited to announce the launch of two new assays to help with the growing epidemic of the COVID-19 virus that is spreading worldwide. The COVID-19 IgG and IgM ELISA’s are successfully validated assays for the qualitative detection of novel coronavirus infected pneumonia cases, suspected clustering cases, and other new coronaviruses in serum or plasma samples (COVID-19) through measurement of the COVID-19 IgM or IgG antibodies.

Assay Background
2019 novel coronavirus (COVID-19) is a single-stranded RNA coronavirus. Comparisons of the genetic sequences of is virus have shown similarities to SARS-CoV and bat coronaviruses. In humans, coronaviruses cause respiratory infections. Coronaviruses are composed of several proteins including the spike (S), envelope (E), membrane (M), and nucleocapsid (N). Results suggest that the spike protein retains sufficient affinity to the Angiotensin converting enzyme 2 (ACE2) receptor to use it as a mechanism of cell entry. Human to human transmission of coronaviruses is primarily thought to occur among close contacts via respiratory droplets generated by sneezing and coughing. IgM is the first immunoglobulin to be produced in response to an antigen and will be primarily detectable during the early onset of the disease.

Assay Principle
These COVID-19 IgG and IgM ELISA assay kits are designed, developed, and produced for the qualitative measurement of the COVID-19 IgM or COVID-19 IgG antibody in serum. The assays utilizes the “IgM capture” or “IgG capture” methods on microplate based enzyme immunoassay technique.

Assay controls and samples are added to the microtiter wells of a microplate that was coated with an anti-human IgM or IgG specific antibodies. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase (HRP) labeled recombinant COVID-19 antigen is added to each well. After an incubation period, an immunocomplex of Anti-hIgM or Anti-hIgG antibody – human COVID-19 IgM or IgG antibody – HRP labeled COVID-19 antigen is formed if there is novel coronavirus IgM or IgG antibody present in the tested materials. The unbound tracer antigen is removed by the subsequent washing step. HRP-labeled COVID-19 antigen tracer bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antigen bound to the coronavirus IgM of IgG on the wall of the microtiter well is proportional to the amount of the coronavirus IgM or IgG antibody level in the tested materials

To learn more, view assay’s here or contact us for questions! 
Novel Coronavirus COVID-19 IgM ELISA Kit
Novel Coronavirus COVID-19 IgG ELISA Kit

Alzheimer’s targets brain cells that help people stay awake

Science News

New Alzheimer’s disease research is providing valuable insight into the cause of the drowsiness associated with dementias. A study performed in a neuropathology lab at UC San Francisco found the brain stem and hypothalamus to be packed with tau protein. The brain stem and hypothalamus oversee several nervous system functions, including keeping people awake and focused. Tau forms “tangles” inside of nerve cells and hinder them from performing necessary functions. In studying three areas of theses structures that help keep people awake, researchers found over 70 percent of the nerve cells were destroyed by tau. These findings will help to drive a greater focus on the brain stem in dementia and Alzheimers research.

Read the full article here.

Eagle Biosciences offers a sensitive and reliable assay for detection of tau in cell culture supernatant, serum, cell culture extracts, plasma, cerebral spinal fluid. We also offer several Tau monomer and preformed fibril products.

Tau ELISA Assay Kit

Active Human Recombinant Tau (K18), P301L mutant Protein Monomer

Active Human Recombinant Tau441 (2N4R) mutant Protein Monomer

Active Human Recombinant Tau (K18), P301L mutant PPF

Active Human Recombinant Tau441 (2N4R), P301S mutant PPF

If you are looking for a specific tau protein related product or if you have questions about our offerings, contact us here.

Eagle Biosciences will be at ASN Kidney Week in Washington, DC!

ASN Kidney Week is this week, Thursday, November 7 to Sunday, November 10, at the Walter E. Washington Convention Center. Stop by booth #2005 to learn more about Vanin-1, Klotho, Intact FGF23 and other assays that could help you with your kidney related research! We’ll be there to answer any questions you may have, or stop and say hi! We love seeing our customers!


Product Highlights

Vanin-1: Eagle Bio has two new Vanin-1 ELISA Kits. Vanin-1 is an early biomarker of renal tubular damage in drug-induced acute kidney injury, obstructive nephropathy, and diabetic nephropathy.

FluoBolt Klotho: This newest addition to our FluoBolt line of fluorescence immunoassays allows for highly sensitive measurement of Klotho, a critical biomarker for chronic kidney disease and failure, renal- and hepatocellular carcinomas, osteoporosis or cardiovascular diseases.

Intact FGF23: This CLEIA ELISA is a unique and highly accurate assay for the measurement of FGF23, which has been linked to impaired renal function.


Contact us for more information about these products.

What is Vanin-1?

Vanin-1 is an epithelial ectoenzyme activating the conversion of pantetheine into pantothenic acid (vitamin B5) and cysteamine and thus is involved in the regulation of oxidative stress and inflammation.

Why is Vanin-1 important?

The highest levels of Vanin-1 expression is assigned to renal tubular epithelial cells. Hence, Vanin-1 released from renal cells can be detected in the urine. Pre-clinical and clinical studies demonstrate that Vanin-1 is an early biomarker of renal tubular damage in drug-induced acute kidney injury, obstructive nephropathy, and diabetic nephropathy. In fact, Vanin-1 has as superior predictive value for acute kidney injury than established markers KIM-1, NGAL, or NAG .

Comparison of human urine Vanin-1 values in apparently healthy individuals and individuals with kidney disease:

Areas of Interest:

  • Acute kidney injury
  • Diabetic nephropathy
  • Drug-induced acute kidney injury
  • Obstructive nephropathy

Assay Highlights:

  • Optimized for human urine samples
  • Highly SPECIFIC and DEFINED characterized antibodies
  • RELIABLE rigorously validated according to FDA guidelines​
  • QUICK one-step ELISA

Eagle Biosciences offers the first fully validated human Vanin-1 assays in a simple-to-use ELISA:

Human Vanin-1 (urine) ELISA Assay Kit

Mouse/Rat Vanin-1 ELISA Kit

Contact us for more information about our Vanin-1 related assay kits.


Eagle Biosciences will be at ASN Kidney Week in Washington, DC!

Stop by booth #2005 to learn more about Vanin-1, Klotho, Intact FGF23 and other assays that could help you with your kidney related research! We’ll be there to answer any questions you may have, or stop and say hi! We love seeing our customers!

The Eagle Biosciences MedFrontier FGF23 (Human Intact FGF23) CLEIA ELISA Assay was used in a recently published study looking at the association between FGF23 levels and major adverse cardiovascular events (MACE) and mortality in patients with type 2 diabetes and normal or mildly impaired kidney function.

FGF23 is an endocrine hormone involved in vitamin D metabolism and phosphorous level regulation. Previous studies have shown a link between insulin resistance and an increase in FGF23, which researchers believe affects renal phosphorous handling and leads to a higher risk of MACE.

The study did find evidence of a connection with FGF23 levels and an increased risk of MACE or mortality in participating patients.

Read more about this study here.

Contact us for more information about this kit.

Study Citation:
Fibroblast Growth Factor 23 and Mortality in Patients With Type 2 Diabetes and Normal or Mildly Impaired Kidney Function
Stanley M.H. Yeung, S. Heleen Binnenmars, Christina M. Gant, Gerjan Navis, Ron T. Gansevoort, Stephan J.L. Bakker, Martin H. de Borst, Gozewijn D. Laverman
Diabetes Care Sep 2019, dc190528; DOI: 10.2337/dc19-0528