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Eagle Biosciences, Inc. is excited to collaborate with Austrian biotech Fianostics, to offer a new fluorescence-based detection platform for immunoassays, called FluoBolt™, which enables highly sensitive assays with high reproducibility and reliability. This dramatically improves the informative value of research with biomarkers.

So far, Fianostics FluoBolt™ Metal Enhanced Fluorescence Immunoassays have been developed to detect

What Makes These Assays Different?

Fianostics FluoBolt™ Metal Enhanced Fluorescence Immunoassays have been established to eliminate the complicated processes that come with your standard sandwich ELISA. By using their FluoBolt™ Metal Enhanced Fluorescence Immunoassays, you can expect

  • High Sensitivity!
  • Single Step Assay Procedure!
  • No Enzyme Substrate!
  • Stable Signal over Time!
  • 100% Compatibility with 96-well ELISA Format!

About Fianostics

Fianostics combines scientific and technical expertise in diagnostics with the development of a high-tech detection platform that benefits in reproducibility from the excellent expertise of Sony DADC BioSciences in the production of polymer consumables for the diagnostic industry. Their know-how is not limited to the development of the new detection platform, but we also provide the diagnostic application with the appropriate immunoassays for specific clinical areas. (for research use only in the US)

About Metal Enhanced Fluorescence

Metal Enhanced Fluorescence (MEF) offers the possibility to dramatically increase the analytical sensitivity of systems based on fluorescence detection. MEF is based on the fact that excitation light interacts with the electrons of metal nano–structures thus generating very high electromagnetic fields (Localised Surface Plasmons, LSPs ) Therefore, such structures are also called
”plasmonic structures” and the combination of (e.g, polymeric) support and structure is known as “plasmonic substrate”. These LSPs lead to an increase in emission output of fluorescent molecules (e.g. fluorescently labelled antibodies) when bound to surfaces with suitable nano-metal structures that can enhance the signal more than 100 times.

Hello Loyal Customers!

Our team here at Eagle Biosciences, Inc. are so excited to introduce our new look for you to enjoy! The new EagleBio.com comes chocked-full of new features that will make your browsing and shopping experience far superior to what you’re used to!

  • Speak with one of our team members online when you need help
  • Fast, easy and secure check out
  • Smooth navigation while browsing one of our many unique products

But That’s Not All…

Not only have we made your shopping experience better when you choose to order from Eagle Biosciences, but we are also working with some phenomenal new partners to bring you new and unique products! Here’s a sneak peak…

  • Fianostics FluoBolt™-FIAs : Metal enhanced fluorescence assays that offers a higher sensitivity, single-step alternative to your basic ELISA. Available for detection of Noggin, Asporin, and Periostin! As well as services to test a variety of other markers!
  • osteomiR testing by TAmiRNA : Workflow that detects 11 microRNA associated with bone quality. We will feature osteomiR Test Kit, as well as services that can run your samples for you.
  • New and unique Drug Monitoring Assays

As always, we appreciate your business! Without you we wouldn’t be able to do what we love by providing you the products you need to change the future! Be sure to keep an eye out for more to come.


If you want to see anything from Eagle Biosciences (products, services, etc.) Let us know! We’re here for you!

Eagle Biosciences Histamine ELISA Assay Kit used in a recent study. This kit is part of our Immunology Assay Kit line which is a line of highly sensitive and specific kits used for the detection of a variety of samples in serum, plasma, tissue, urine, and saliva.

Histamine deficiency aggravates cardiac injury through miR-206/216b-Atg13 axis-mediated autophagic-dependant apoptosis

Abstract:

Histamine is a widely distributed biogenic amine involved in the regulation of an array of biological processes. Serum histamine level is markedly elevated in the early stages of acute myocardial infarction, whereas the role it plays remains unclear. Histidine decarboxylase (HDC) is the unique enzyme responsible for histamine production, and cardiac injury is significantly aggravated in HDC knockout mice (HDC−/−), in which histamine is deficient. We also observed that autophagy was highly activated in cardiomyocytes of HDC−/− mice post acute myocardial infarction (AMI), which was abolished by compensation of exogenous histamine. The in vivo and in vitro results showed that acting through histamine 1 receptor, histamine increased miR-206 and miR-216b, which worked in concert to target to Atg13, resulting in the reduction of autophagy activation under hypoxia and AMI condition. Further study revealed that Atg13 interacted with FADD to promote the activation of caspase-8 and cell apoptosis. Taken together, these data unveil a novel intracellular signaling pathway involved in histamine regulating myocardial autophagy and apoptosis under hypoxia and AMI condition, which might help to more comprehensively evaluate the usage of histamine receptor antagonists and to develop new therapeutic targets for myocardial infarction.

Ding, Suling, et al. “Histamine Deficiency Aggravates Cardiac Injury through MiR-206/216b-Atg13 Axis-Mediated Autophagic-Dependant Apoptosis.” Cell Death & Disease, vol. 9, no. 6, 7 June 2018, p. 694., doi:10.1038/s41419-018-0723-6.

Eagle Biosciences announces the expansion of our line of Gastrointestinal Assays to include a Lactoferrin ELISA Assay Kit as well as a TNF-Alpha Stool ELISA Assay Kit. These kits add to our world class line of stool assays that include ZonulinPancreatic ElataseBlastocystis, and NGAL

Lactoferrin ELISA Assay TNF-Alpha ELISA Assay
Size: 1×96 wells Size: 1×96 wells
Sensitivity: 0.369 ng/mL Sensitivity: 10 pg/mL
Incubation: 1.5 hours Dynamic Range: 10-500 pg/mL
Sample: Stool Incubation: 4.5 hours
Size: 15 mg Sample: Plasma, Serum, Stool

What is Lactoferrin and TNF-Alpha?

Lactoferrin is a protein found in a monomer or tetramer, that is involved in iron homeostasis, innate defense against microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer. Elevated levels of lactoferrin are associated with Inflammatory Bowel Disease (IBD) such as Ulcerative Colitis (UC) or Crohn’s Disease (CD).

TNF-Alpha’s primary role is the regulation of immune cels. It stimulates apoptotic cell death, cell proliferation, and differentiation, inhibits tumor genesis and viral replication. Elevated levels of TNF-Alpha are found in patients suffering from Crohn’s Disease (CD), Ulcerating Colitis (UC), or Rheumatoid Arthritis (RA).

Eagle Bioscience’s Total Testosterone ELISA Assay Kit was used in a recent study. This kit is part of our line of Steroid Assay Kits which is a line of highly sensitive and specific assays used to detect a variety of samples in serum, plasma, tissue, urine, and saliva.

Impact of resistance training on body composition and metabolic syndrome variables during androgen deprivation therapy for prostate cancer: a pilot randomized controlled trial

Background

Prostate cancer patients on androgen deprivation therapy (ADT) experience adverse effects such as lean mass loss, known as sarcopenia, fat gain, and changes in cardiometabolic factors that increase risk of metabolic syndrome (MetS). Resistance training can increase lean mass, reduce body fat, and improve physical function and quality of life, but no exercise interventions in prostate cancer patients on ADT have concomitantly improved body composition and MetS. This pilot trial investigated 12 weeks of resistance training on body composition and MetS changes in prostate cancer patients on ADT. An exploratory aim examined if a combined approach of training and protein supplementation would elicit greater changes in body composition.

Conclusions

A 12-week resistance training intervention effectively improved sarcopenia, body fat %, strength and quality of life in hypogonadal prostate cancer patients, but did not change MetS or physical function. PRO did not offer additional benefit in improving body composition.

Dawson, Jacqueline K., et al. “Impact of Resistance Training on Body Composition and Metabolic Syndrome Variables during Androgen Deprivation Therapy for Prostate Cancer: a Pilot Randomized Controlled Trial.” BMC Cancer, vol. 18, no. 1, 3 Apr. 2018, p. 368., doi:10.1186/s12885-018-4306-9.

Yale Researchers Identify Target for Novel Malaria Vaccine

Yale University

A team of researchers has developed a vaccine against malaria infection targeting a unique protein produced by malaria parasites. Plasmodium macrophage migration inhibitory factor (PMIF) suppresses the infection fighting cells that respond to threats and protect the body against reinfection. PMIF is critical for the completion of the parasite life cycle, specifically the transmission to new hosts. An RNA based vaccine developed by the researchers protects against reinfection in mice. The next step is to develop a vaccine that could be used for individuals who have never been infected with malaria. Researchers also have noted that the PMIF protein has been conserved over various strains of malaria, and therefore it should be nearly impossible for the virus to develop a resistance to the vaccine. 

Read More.

Why measure zonulin?

The zonulin biomarker is a pre-haptoglobin (pre-HP2) protein found in the gut. It is responsible for the permeability of the mucosal barrier of the intestines. When zonulin binds to the epithelial cells of the intestines a signal cascade is induced. This cascade disassembles the paracellular tight junctions of the intestinal wall. The tight junctions of the intestinal walls are one of two ways that allow molecules to transfer from the gut lumen to the bloodstream and vice versa. When zonulin levels in the gut increase and go out of balance, these junctions open wider and stay open longer. These widened entry points allow larger macromolecules into the bloodstream or into the gut lumen. The molecules that pass through these breaches can increase the body’s natural immune reaction, causing inflammation and a number of autoimmune diseases.

Diseases Associated with increased Zonulin levels:

  • Type-1 Diabetes
  • Rheumatoid Arthritis
  • Celiac Disease
  • Liver Disease 

The Eagle Biosciences 8-Isoprostane ELISA Assay Kit was utilized in a recent research project. This kit is part of our Oxidative Stress Assay Kit line which is a line of highly sensitive and specific assays used to detect a variety of samples in serum, plasma, tissue, urine, and saliva.

Effect of flavoring chemicals on free radical formation in electronic cigarette aerosols

Background

Flavoring chemicals, or flavorants, have been used in electronic cigarettes (e-cigarettes) since their inception; however, little is known about their toxicological effects. Free radicals present in e-cigarette aerosols have been shown to induce oxidative stress resulting in damage to proliferation, survival, and inflammation pathways in the cell. Aerosols generated from e-liquid solvents alone contain high levels of free radicals but few studies have looked at how these toxins are modulated by flavorants.

Conclusions

Our results suggest that flavoring agents play an important role in either enhancing or inhibiting the production of free radicals in flavored e-cigarette aerosols. This information is important for developing regulatory strategies aimed at reducing potential harm from e-cigarettes.

Bitzer, Zachary T, et al. “Effect of Flavoring Chemicals on Free Radical Formation in Electronic Cigarette Aerosols.” Free Radical Biology and Medicine, vol. 112, 20 May 2017, p. 200., doi:10.1016/j.freeradbiomed.2017.10.315.

FGF-23 is a hormone that is secreted by osteoblasts within the bones. This protein works with the kidneys to help regulated levels of phosphate in the blood/serum. The kidney gets rid of excess phosphate by excreting it in urine. When more phosphate is needed, the kidney reabsorb phosphate into the bloodstream. FGF-23 signals the kidneys to stop reabsorbing phosphate into the bloodstream. The fibroblastic growth factor binds to a receptor protein called FGF receptor 1. These receptors span the membrane of kidney cells. The binding of the protein and the receptors stimulates a signal cascade that stops phosphate reabsorption into the bloodstream. 

image credit: http://www.kidney-international.org/

Why Measure FGF-23?

Phosphate plays a critical role in the formation and growth of bones in children and for maintaining bone strength in adults. If there is an imbalance in levels of FGF-23 in the body, this can cause high or low levels of phosphate in the bloodstream. Low levels of phosphate in the blood can result in hypophosphatemia rickets or osteomalacia, which is a weakening of the bones which can cause bone pain and fractures. High levels of phosphate in the blood can indicate kidney dysfunction. 

A next generation cancer therapy helps immune cells detect, and destroy cancer cells. This therapy works by evading two mechanisms that cancer cells use to evade macrophages, cells whose job it is to eat and destroy cancerous cells.

“The researchers [at Brigham and Women’s Hospital] tested the supramolecular therapeutic in animal models of aggressive forms of breast cancer and skin cancer, comparing their drug directly with a drug currently available in the clinic. Mice that were untreated formed large tumors by Day 10. Mice treated with currently available therapies showed decreased tumor growth. But mice treated with the new supramolecular therapy had complete inhibition of tumor growth. The team also reported an increase in survival and a significant reduction in metastatic nodes.”

This treatment is skill in the testing phases but it hold potential for future applications.

Read More.