Author: Eagle Biosciences

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Cov19 FluoBolt-DAT Detects Antibodies Against the Omicron Variant

by Eagle Biosciences


New data suggests the Cov19 FluoBolt-DAT detects antibodies against the new Omicron variant.

Quantitative Duplex Antibody Test (DAT) for antibodies to SARS-CoV-2

The Cov19 FluoBolt-DAT allows the simultaneous and quantitative measurement of antibodies against the S1 RBD AND Nucleocapsid antigen of SARS-CoV-2. In contrast, other antibody assays detect all antibodies generated against the S1- OR NC-protein. This test, however, is epitope specific. It detects immune-dominant antibodies species, i.e. the most important antibodies generated by either vaccination or infection.

This is demonstrated by the fact, that it detects anti-S1RBD against all dominant virus variants identified so far. The data indicates (see chart below) that the S1RBD part of the Cov19 FluoBolt-DAT assay detects the immune-dominant epitope and that individuals testing S1RBD -positive in the FluoBolt-DAT test have developed significant amounts of antibodies against this epitope. Therefore, they might be well protected against all current and future SARS-CoV-2 variants

Cov19 FluoBolt-DAT

Benefits of the Cov19 FluoBolt-Dat Assay:

    • Only One Single Measurement Required
    • Small Sample Size Need – 10 uL
    • Results within 60 minutes

    Other Cov19 FluoBolt-DAT Resources:

    Check out our other COVID-19 assays here.

    If you have any questions about this kit or our other offerings, contact us here.

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GA-Map Dysbiosis Test Lx Technical Spotlight

by Eagle Biosciences

GA-map Technical Spotlight

This week Eagle Biosciences is highlighting a revolutionary test in the microbiome industry. The GA-map® Dysbiosis Test Lx was developed by Genetic Analysis in Norway. The GA-map ® is a tool that allows mapping of the intestinal microbiota profile for a selected set of bacteria in a sample. The test is based on advances in DNA profiling using probes targeting variable regions (V3 to V7) of the bacterial 16S rRNA gene to characterize and identify bacteria present. The targets are identified in a molecular multiplex assay that utilizes the Single Nucleotide Primer Extension (SNuPE) technology patented by professor Knut Rudi of the Norwegian University of Life Sciences (​NMBU) (US6617138). A unique algorithm takes advantage of all the data generated by the detection of the SnuPE products to determine the microbiota profile in the sample. The algorithm is incorporated in the GA-map® Dysbiosis Analyzer software that accompanies the test.

Check out this video for a quick overview of the GA-map ® Dysbiosis Test Lx Platform equipment and techniques.

Note: This test is for research use only and not to be used for diagnostic procedures

If you have any questions about the GA-map® Dysbiosis Test Lx or our other offerings contact us here.

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iLite Assay Ready Cells Product Spotlight

by Eagle Biosciences

iLite Assay Ready Cells Product Spotlight

One of our supplier’s was recently featured in an editorial by Select Science! Svar Life Sciences has been working closely with Dr. Erik Tillman at Akero Therapeutics. Tillman and his team are developing a multi-modal drug that could help treat the drivers of non-alcoholic steatohepatitis (NASH), a severe form of non-alcoholic related fatty liver disease (NAFLD) that has increased risk for cardiovascular and liver related morbidity and mortality. Him and his team have been working with Svar to create custom iLite assay-ready cells for the cell-based assays that have become a key role in the work Tillman and his teammates perform daily.

“The assay-ready cells have been much more robust and the results have been more reproducible than other cell-based assays that we had tried to use or develop in the past,” says Tillman. “Using the assay-ready cells from Svar, we understand how the cells have been made and characterized, and we are confident in how the cells are going to perform from one vial to the next, and from one lot to the next. Svar’s expertise in the engineering of the cell system and our results thus far have given us the confidence to broaden our use of the assay-ready cells.”

Click here to read the full editorial about Dr. Tillman’s research and the role of Svar’s iLite assay-ready cells.

If you have any questions about Svar’s iLite Assay Ready Cells or our other offerings contact us here.

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Biomarker Spotlight: Endostatin

by Eagle Biosciences

Endostatin Biomarker Spotlight
Endostatin is an endogenous angiogenesis inhibitor localized in the vascular basement membrane in various organs. The biological functions of the endostatin-network involve SPARC, thrombospondin-1, glycosaminoglycans, collagens, and integrins. Endostatin is expressed during the progression of renal fibrosis in tubular cells of injured tissue.

A recent study aimed to discover the role of endostatin in in the dysregulation of angiogenesis in patients with kidney and heart failure. Scientists found that due to its capability to inhibit angiogenesis and tumor growth, endostatin is highly involved in these processes in chronic kidney disease and heart failure. Since endostatin is not just a biomarker of angiogenesis but also a hormone regulating these processes, pharmacologic intervention in this system might offer new therapeutic options in the future.

Li M, Popovic Z, Chu C, Krämer B, K, Hocher B: Endostatin in Renal and Cardiovascular Diseases. Kidney Dis 2021. doi: 10.1159/000518221 Full Text Here.

Eagle Biosciences offers a robust assay for the measurement of endostatin in human samples, the Endostatin ELISA Assay Kit. The benefits of this assay include:

  • for serum, plasma, and urine samples
  • SIMPLE analysis – results in 4.5 h
  • LOW SAMPLE VOLUME – only 20µl sample / well
  • HIGH QUALITY – fully validated assay according to ICH/FDA/EMEA

We also offer a Mouse/Rat Endostatin ELISA Assay Kit for other testing needs.

If you have any other questions about these products or our other offerings, contact us here.

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Eagle Biosciences will be at AACC in Georgia!

by Eagle Biosciences

EagleBio Visits AACC in Atlanta Georgia

Eagle Biosciences will be at AACC in Atlanta Georgia!

AACC is next week, Sunday September 26th – Thursday September 30th, at the Georgia World Congress Center. Stop by booth #3036 to learn more about the GA-Map Dybiosis Test Lx and Cov19 FluoBolt-DAT and other assays that could help you with your microbiome or COVID-19 research! We will be there to answer any questions you may have, or stop and say hi! We love seeing our customers!

Product Highlights

GA-Map Dysbiosis Test Lx: The first and only standardized solution for microbiome profiling! The GA-Map Dysbiosis Test Lx is a simple multiplex stool assay that identifies 48 bacterial markers. All reagents and required software are included in the kit, and it is designed to be run for routine testing.

Cov19 FluoBolt-DAT: This newest addition to our FluoBolt line of assays is a duplex antibody test. This assay is intended for the simultaneous detection of anti-nucleocapsid antibodies and anti-S1 RBD antibodies to SARS-CoV-19. This is a valuable tool for evaluating immunity against SARS-CoV-2 acquired through infection as well as vaccination.

If you have any other questions about these products or our other offerings, contact us here.

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Vanin-1 Biomarker Spotlight

by Eagle Biosciences

Vanin-1 Biomarker Spotlight

Vanin-1 (VAN1) is an anchored protein that catalyzes the hydrolysis of pantetheine to pantothenic acid (vitamin B5) and cyteamine. VAN1 has a broad tissue expression with the highest levels being observed in kidney tubular epithelial cells. The GPI anchor of VAN1 can be cleaved by a yet unknown mechanism, resulting in VAN-1 being shed into the extracellular space.

Vanin-1 plays a pivotal role in oxidative stress and the inflammatory response. However, its relationship with traumatic sepsis remains unknown. In a study aimed to identify whether VAN1 could be used as a biomarker for traumatic sepsis, scientists found there is a significant relationship between plasma VAN1 and sepsis in both the internal test cohort and the external validation cohort. These results contribute to the body of evidence supporting the use of plasma VAN1 in the early prediction of traumatic sepsis.

Learn more about that study here.

Eagle Biosciences offers a comprehensive Vanin-1 ELISA for urine samples.

Vanin-1 ELISA Assay Highlights

  • Optimized for human urine samples
  • Highly SPECIFIC and DEFINED characterized antibodies
  • RELIABLE – rigorously validated
  • QUICK one-step ELISA

We also provide a Vanin-1 Mouse/Rat ELISA Assay Kit that has been validated for mouse or rat serum, plasma, or urine samples.

If you have any questions about this product or our other offerings, contact us here.

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New Cov19 FluoBolt-DAT Test!

by Eagle Biosciences

At this time it is unknown for how long antibodies persist following infection or vaccination, and if the presence of antibodies confers protective immunity. Antibodies to SARS-CoV-2 are generally detectable in blood several days after initial infection or vaccination, although the duration of time antibodies are present post-infection or post-vaccination is not well characterized. Individuals may have detectable virus present for several weeks following seroconversion.

The Cov19 FluoBolt-DAT test, manufactured in Austria by Fianostics GmbH, is a duplex antibody test that is intended to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection or vaccination. There is a high demand to know how protected populations are after COVID-19 onset or vaccination, this test is the next step in identifying immunity.

How the Cov19 FluoBolt-DAT Test Works

When performing the assay, 10 µl of a patient serum or plasma sample are simultaneously mixed with 50 µl of two different fluorescence-labeled tracer antibodies against the S1RBD and against the NC protein and incubated in our special fluorescence-enhancing microtiter plate for 60 minutes. By measuring at 2 different wavelengths, their displacement by homologous antibodies from the patient sample is determined simultaneously. The signals at one wavelength provide information about the concentration of anti-S1RBD antibodies. The signals at the other wavelength indicate the concentration of anti-nucleocapsid antibodies.

By using the supplied calibrators, a calibration curve for the anti-S1RBD and the antiNC antibodies is constructed, and the antibody concentration of a patient sample is read from it. With just a single measurement, this results in a quantitative determination of these
two antibody species against SARS-CoV-2, which represent the majority of the immune response and may protect against infection and illness.

Cov19 FluoBolt-DAT Test Standard Curves
For measurement, any commercially available fluorescence microtiter plate reader can be used.

Click here to learn more about the Cov19 FluoBolt-DAT test.

If you have any questions about this kit or our other offerings, contact us here.

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PTX3 Biomarker spotlight

by Eagle Biosciences

PTX3 Biomarker Spotlight
Pentraxins are a superfamily of acute phase reactants characterized by a pentameric structure. Pentraxin 3 (PTX3), is locally produced and released by a variety of cell types including macrophages, neutrophils, myeloid-derived mesangial cells, synovial cells, smooth muscle cells, alveolar epithelium, and glial cells. PTX3 is induced in response to either inflammatory cytokines interleukin-1 β (IL-1 β) and tumor necrosis factor α (TNFα) or the selected associated molecular patterns (PAMPs). PTX3 is elevated in critically ill patients, with a gradient from systematic inflammatory response syndrome to septic shock, and in several other diseases, such as myocardial infarction, rheumatoid arthritis, atherosclerosis, small vessel vasculitis and psoriasis.

Oncologists at Queen Mary’s University whether PTX 3 could be a reliable biomarker for detection of pancreatic ductal adenocarcinoma (PDAC). PDAC is characterized by an intense desmoplastic stroma, laid down by the pancreatic stellate cells (PSC). One of the defining features of PDAC is that there are very few cancer cells. Pancreatic cancer is surprisingly made up of mostly non-cancer cells, which have been co-opted by cancer to build a huge amount of scar tissue or stroma around the cancer, providing a strong defense for the cancer cells. The researchers found that PTX3 is a putative stromally-derived biomarker for PDAC which warrants further testing in prospective, larger, multi-center cohorts and within clinical trials targeting stroma.

To read more about this study, click here.

Related Products

Pentraxin-3 ELISA Assay Kit
High Sensitive CRP ELISA Assay Kit
SAP ELISA Assay Kit

If you have any questions or want to learn more about our offerings, contact us here.

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Cortisol Saliva ELISA Assay Kit Product Announcement

by Eagle Biosciences

Cortisol ELISA Assay Kit

Eagle Biosciences is excited to announce the new Cortisol Saliva ELISA Assay Kit. The kit is intended for the quantitative determination of cortisol in human saliva.

Why Measure Cortisol?

Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and external administration of ACTH. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1–2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva, salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.

Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia and carcinoma.

Check out the benefits of Cortisol Saliva ELISA Assay Kit:

  • NO SHAKING required
  • ROOM TEMPERATURE incubation
  • SMALL SPECIMEN SAMPLE required

If you have any questions about this kit or our other offerings, contact us here.

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ACTH ELISA Assay Kit Highlighted in Recent Publication

by Eagle Biosciences

The Eagle Bioscience’s ACTH ELISA Assay Kit was recently highlighted in a publication about the cortisol and adrenocorticotrophic hormone (ACTH) in infants after receiving corticosteroids following cataract surgery.

Abstract

Purpose
Cushingoid features are occasionally encountered in infants after pediatric cataract surgery. The aim of this study is to evaluate whether the use of topical glucocorticoids (GCs) following congenital cataract surgery can result in endogenous adrenal suppression and/or systemic side effects similar to those seen with systemic steroids.

Methods
A prospective study was performed on 20 infants with bilateral congenital cataract. All infants received a single subconjunctival betamethasone injection of 1 mg at the end of surgery in addition to topical dexamethasone eye drops 1 mg/ml for 6 weeks. All infants had anthropometric measurements and blood pressure measurements, serum cortisol, and ACTH level measurements before surgery and 2 months after. In addition, the total administered glucocorticoid adjusted per weight was calculated.

Results
The mean age of the infants was 4.93 ± 2.58 months. Thirteen were males (65%). The total administered glucocorticoid dose was 18.7 mg and the mean cumulative dexamethasone equivalent dose administered was 2.75 ± 1.31 mg/kg. There was a statistically significant increase in the adjusted weight percentile for age (P = 0.009). Both the systolic and diastolic blood pressure were significantly elevated (P = 0.005 and P = 0.025 respectively). There was a statistically significant reduction in both the morning and afternoon serum ACTH levels (P = 0.023 and P = 0.014). The reduction in serum cortisol levels was statistically non-significant.

Conclusions
Topical steroids following pediatric cataract surgery can result in both subclinical and clinical changes in the hypothalamic–pituitary–adrenal axis that can be easily overlooked and need careful attention and follow-up.

Aly, A., Gouda, J., Awadein, A. et al. Serum cortisol and adrenocorticotrophic hormone (ACTH) in infants receiving topical and subconjunctival corticosteroids following cataract surgery. Graefes Arch Clin Exp Ophthalmol (2021). https://doi.org/10.1007/s00417-021-05221-0

If you have any questions about this product or our other offerings, contact us here.