ACTH ELISA Assay
ACTH ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 1.0 pg/mL
Dynamic Range: 11 – 416 pg/mL
Incubation Time: 2.5 hours
Sample Type: Plasma
Sample Size: 200 µL
Alternative Names: Adrenocorticotrophic Hormone ELISA, Human ACTH ELISA
For Research Use Only
EDTA plasma samples from normal healthy adults ages 20 – 60 were collected and measured with this ELISA. The recommended normal range for ACTH concentration by using this ELISA is between 1 – 72 pg/mL. We strongly recommend for each clinical laboratory to establish its own normal range by measuring EDTA plasma samples with this ELISA. Please note that sample collection time of the day may have impact on the ACTH normal range.
The Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human ACTH in EDTA-plasma sample. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to N-terminal and C-terminal epitopes of ACTH.
Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to the N-terminal of human ACTH. Immediately, a horseradish peroxidase (HRP) conjugated anti C-terminal of human ACTH antibody is added to each well. After the first incubation period, a “sandwich” of solid-phase monoclonal antibody – human ACTH – HRP conjugated antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human ACTH in the test sample. A standard curve is generated by plotting the absorbance versus the respective human ACTH concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human ACTH in test samples is determined directly from this standard curve.