Cortisol Saliva ELISA Assay Kit

$190.00

The Eagle Biosciences Cortisol Saliva ELISA Assay Kit is intended for the quantitative determination of Cortisol in human saliva by an enzyme immunoassay. The Cortisol Saliva ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: CRT32-K01 Categories: , , ,

Cortisol Saliva ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 1 ng/mL
Dynamic Range: 1–100 ng/mL
Incubation Time: 90 minutes
Sample Type: Saliva
Sample Size: 50 µL

Controls Included

Additional Information

Assay Background


Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia and carcinoma.

Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1–2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva, salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.

Assay Principle


The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of cortisol in the sample. A set of standards is used to plot a standard curve from which the amount of cortisol in patient samples and controls can be directly read.

Typical Standard Curve


Manual

Product Manual