Cortisol Saliva ELISA Assay Kit


The Eagle Biosciences Salivary Cortisol ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol concentration in saliva. The Cortisol Saliva ELISA Assay kit is intended for research use only.

SKU: DCMO020 Categories: , ,

Cortisol Saliva ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.12 ng/ml
Dynamic Range: 0.5 -100 ng/ml
Incubation Time: 1.5 hour
Sample Type: Saliva
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

For SI Units: ng/mL x 2.76 = nmol/L

Reference Values (Normal):
AM Values           (median= 6.3 ng/ml)     0.72 – 13.6 ng/ml  (N=50)
PM Values           (median=1.77 ng/ml)     0.32 – 5.14 ng/ml  (N=50)

It is suggested that each laboratory establish its own normal ranges.

Additional Information

Assay Background

Cortisol is a steroid hormone released from the adrenal cortex in response to a hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system.  Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form.

The majority of cortisol in saliva is not-bound and enters the saliva via intracellular mechanisms. Salivary cortisol levels are unaffected by salivary flow rate or salivary enzymes.  It is a high correlations between serum and saliva cortisol levels.  These normal endogenous functions are the basis for the physiological consequences of chronic stress – prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune/ inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.

Assay Principle

The Cortisol (antigen) in the sample competes with the antigenic Cortisol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Cortisol coated on the microplate (solid phase) of the Cortisol Saliva ELISA Assay Kit.  After incubation, the bound/free separation is performed by a simple solid-phase washing.  Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution (H2SO4) is added.  The colour intensity is inversely proportional to the Cortisol concentration of in the sample.  Cortisol concentration in the sample is calculated through a calibration curve.


Product Manual



Flakoll, B;Ali, AB;Saab, CY;, (2017). Twitching in veterinary procedures: How does this technique subdue horses? Journal of Veterinary Behavior: Clinical Applications and Research, 1823-28.

Schmitt, B et. al.  Circadian rhythms of ewes suckling singletons versus twins during the second week of lactation. BIOS 2014. 85(4):207-217. doi:

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