At this time it is unknown for how long antibodies persist following infection or vaccination, and if the presence of antibodies confers protective immunity. Antibodies to SARS-CoV-2 are generally detectable in blood several days after initial infection or vaccination, although the duration of time antibodies are present post-infection or post-vaccination is not well characterized. Individuals may have detectable virus present for several weeks following seroconversion.

The Cov19 FluoBolt-DAT test, manufactured in Austria by Fianostics GmbH, is a duplex antibody test that is intended to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection or vaccination. There is a high demand to know how protected populations are after COVID-19 onset or vaccination, this test is the next step in identifying immunity.

How the Cov19 FluoBolt-DAT Test Works

When performing the assay, 10 µl of a patient serum or plasma sample are simultaneously mixed with 50 µl of two different fluorescence-labeled tracer antibodies against the S1RBD and against the NC protein and incubated in our special fluorescence-enhancing microtiter plate for 60 minutes. By measuring at 2 different wavelengths, their displacement by homologous antibodies from the patient sample is determined simultaneously. The signals at one wavelength provide information about the concentration of anti-S1RBD antibodies. The signals at the other wavelength indicate the concentration of anti-nucleocapsid antibodies.

By using the supplied calibrators, a calibration curve for the anti-S1RBD and the antiNC antibodies is constructed, and the antibody concentration of a patient sample is read from it. With just a single measurement, this results in a quantitative determination of these
two antibody species against SARS-CoV-2, which represent the majority of the immune response and may protect against infection and illness.

Cov19 FluoBolt-DAT Test Standard Curves
For measurement, any commercially available fluorescence microtiter plate reader can be used.

Click here to learn more about the Cov19 FluoBolt-DAT test.

If you have any questions about this kit or our other offerings, contact us here.