This GFAP ELISA Assay kit is for quantitative determination of GFAP (Glial Fibrillary Acidic Protein) in human serum or Cerebrospinal Fluid (CSF). The GFAP ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

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GFAP ELISA Assay Kit developed and Manufactured in the USA

Size:  1×96 wells
Sensitivity:  10 pg/ml
Dynamic Range:  62.5 – 4000 pg/mL
Incubation Time:  3 hours
Sample Type:  Serum, CSF
Sample Size: 50 µL
Alternative Names:: Glial Fibrillary Acidic Protein
For Research Use Only

Key Benefits of this assay

  • Easy to run GFAP assay
  • Run both Serum and CSF samples
  • The MOST SENSITIVE GFAP ELISA available in the world
  • Simple ELISA Reader is all that is needed

Assay Principle

The GFAP ELISA Assay Kit test is based on the principle of a solid phase enzyme-linked sandwich immunosorbent assay. The assay system utilizes a specific monoclonal antibody directed against a distinct antigenic determinant on the GFAP molecule and is coated on the microtiter wells for the solid phase immobilization of GFAP. A biotin labeled rabbit anti-GFAP antibody is used as a reporter molecule in a sandwich immunoassay and streptavidin conjugated to Horse radish peroxidase (HRP) is used as the detector molecule. The test sample (serum) is allowed to react with the capture antibody which immobilizes GFAP present in the sample. Following washing, biotinylated polyclonal reporter antibody is added to the wells resulting in the GFAP molecule being sandwiched between the solid phase and biotin-labeled antibodies. After an additional incubation with streptavidin-HRP the wells are washed, a TMB substrate solution is added and the relative absorbance units (AU) are measured spectrophotometrically using a microtiter plate reader at 450nm. The concentration of GFAP is directly proportional to the AUs of the test sample and is determined from the standard curve.

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Additional Information

Assay Background

Glial Fibrillary Acidic Protein (GFAP) is a member of the intermediate filament proteins found in the astroglial cells of the Central Nervous System (CNS).The quantitation of GFAP in serum or CSF levels is recognized as a method in the diagnosis of injury to brain. During the injury to brain or spinal cord, GFAP is released into serum and CSF within a few hours after the injury, and shown to be a biomarker for Traumatic Brain Injury (TBI) and retinal stress.

Typical Standard Curve


Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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