Tag: Coronavirus

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Studies Show that Neuropilin-1 Could Play Role in Transmission of COVID-19

by Eagle Biosciences
Studies Show that Neuropilin-1 Could Play Role in Transmission of COVID-19

Eagle News

Two Independent Research Teams Find Possible Link Between Neuropilin-1 and SARS-CoV-2 Transmission

Neuropilin-1 (NRP1) is an essential transmembrane cell surface receptor acting primarily as a co-receptor for various ligands (i.e. VEGF, Semaphorins). Due to alternative splicing or shedding, the extracellular region can be released into circulation as soluble Neuropilin-1. NRP1 functions in many key biological processes including the neuronal, cardiovascular and the immune system. The virus SARS-CoV-2 is the causative agent of the coronavirus disease COVID-19 NRP1 is expressed in multiple cell types in the body but occurs primarily on cells in the lung, nose and brain (i.e. respiratory and olfactory epithelium as well as in the CNS). SARS CoV 2 enters the host cells by its spike proteins mainly through its binding to the angiotensin converting enzyme 2 (ACE2).

Click here for summary of findings.

Studies:

Neuropilin-1 facilitates SARS-CoV-2 cell entry and provides a possible pathway into the central nervous system. Cantuti-Castelvetri L et al., Science 13 Nov, 2020; Vol. 370, Issue 6518, pp. 856-860. Full publication.

Neuropilin-1 is a host factor for SARS-CoV-2 infection. Daly JL et al., Science, 13 Nov 2020; Vol. 370, Issue 6518, pp. 861-865. Full publication.

About the Neuropilin-1 ELISA:

  • Only assay that measures free and ligand bound soluble NRP1
  • Low sample volume – only 10µl needed
  • Highly specific using epitope mapped antibodies
  • Rigorously validated according to international guidelines

Contact us for more information about this or our other SARS-CoV-2 related products.

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How Does COVID-19 Effect Your Central Nervous System?

by Eagle Biosciences

A new article published by Nelly Kanberg, et al. titled Neurochemical evidence of astrocytic and neuronal injury commonly found in COVID-19 has set out to study how COVID-19 impacts the central nervous system. It’s already known how this novel coronavirus effects the respiratory system, but does the body’s inflammatory response to this virus cause lasting effects to the nervous system?

For this study, two biomarkers in human plasma were measured; neurofilament light chain protein (NfL) and glial fibrillary acidic protein (GFAP). The samples came from patients who had mild, moderate or severe cases of COVID-19 (n=47). Of those 47 patients, the ones who had a severe case of COVID-19 showed higher concentrations of GFAP and NfL.

Glial Fibrillary Acidic Protien, or GFAP for short, is a known biomarker in the central nervous system (CNS) that is typically studied in those with brain injuries. When a brain injury occurs (concussion, retinal stess, tumors, etc.), GFAP is released into the blood stream and helps determine the severity of the injury. The results of this study help support and determine the relationship between COVID-19 and potentially lasting neural injuries.

To read this article in full, click here.

To learn more about how to measure GFAP, click here or contact us with your questions

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Comparison of SARS-CoV-2 antibody assays

by Eagle Biosciences

Comparison of the Elecsys® Anti-SARS-CoV-2 immunoassay with the EDITM enzyme linked immunosorbent assays for the detection of SARS-CoV-2 antibodies in human plasma

Both of Epitope Diagnostics’ Anti-SARS-COV-2 antibody assays (IgG and IgM) have been featured in a head-to-head comparison against Elecsys® Anti-SARS-CoV-2. Elecsys® Anti-SARS-CoV-2 is manufactured by Roche Diagnostics and has been the gold-standard in the diagnostics industry for automated assays.

Abstract: Recently, Roche Diagnostics (Rotkreuz, Switzerland) has launched the IVD CE-marked Elecsys® Anti-SARS-CoV-2 assay for the qualitative detection of SARS-CoV-2 antibodies on the cobas e immunoassay analyzers. The aim of this study was to compare the clinical performance of the Elecsys® Anti-SARS-CoV 2 assay with the EDITM SARS-CoV-2 IgM and IgG enzyme linked immunosorbent assays (ELISA), which we have recently established in our laboratory.

Read and Learn More Here

Coronavirus COVID-19 IgG ELISA Assay

Coronavirus COVID-19 IgM ELISA Assay Kit

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Expanded COVID-19 Assay Selection

by Eagle Biosciences

We, at Eagle Biosciences, Inc. are rapidly expanding our selection of Coronavirus Assays by welcoming two new research kits! With the ongoing pandemic, it’s more important now than ever that we continue to offer the highest quality assays to our customers. Here are our newest additions;

Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay
Catalog Number: E111

The Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay Kit is manufactured in Germany by Mediagnost. This assay is a highly specific enzyme immunoassay for the detection of IgG antibodies directed against SARS-CoV-2-S1 Receptor Binding Domain (RBD) in human blood. In this assay the recombinant Receptor Binding Domain (RBD) of SARS-CoV-2 S1 spike protein, which binds the ACE2 receptor, is used. The use of RBD increases the specificity of the assay since the domain is identical with SARS-CoV but not with MERS-CoV for example. Antibodies directed against the RBD neutralize both virus strains SARS-CoV and SARS-CoV-2.

Size: 1×96 wells
Incubation Time: 2 hours 40 minutes
Sample Type: Serum and Plasma
Sample Size: 100 µl
Controls Included

To learn more about the Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay click here*

Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit
Catalog Number: 3940

The Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is manufactured in Germany by Generic Assays. This assay is a module based Enzyme Immunoassay for the confirmation of positive IgG antibodies against SARS coronavirus 2 (SARS-CoV-2) in the first screening. The Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit determines the specificity of antibodies against the main immunodominant antigens (Spike Glycoprotein 1, Spike Glycoprotein 2, Nucleocapsid) of SARS-CoV-2 in human serum or plasma. This test kit consists of modules separately coated with the major antigens of the virus as seen in the illistration below:

Size: 96 wells (24 samples x 4)
Incubation Time: 2 hours
Sample Type: Serum or Plasma
Number Of Tests Per Kit: 24 (22 samples + controls)
Sample Size: 50 µl
Controls Included

To learn more about the Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit click here*

 

Check out our entire Coronavirus Series here or contact us with any questions or inquires

 

*These kits are for research use only and should not be used for diagnostic procedures.