Coronavirus COVID-19 IgG ELISA Assay


This Coronavirus COVID-19 IgG ELISA Assay Kit is used for the qualitative detection of novel coronavirus infected pneumonia cases, suspected clustering cases, and other new coronaviruses in serum samples (COVID-19) through measurement of the COVID-19 IgG antibody. It is a serological plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. This test is not the same as PCR or Rapid Test. This Coronavirus COVID-19 IgG ELISA Assay Kit is for Research Use Only (RUO) in the USA, CE-Marked.

Coronavirus COVID-19 IgG ELISA Assay

Coronavirus COVID-19 IgG ELISA Assay was developed and manufactured in the US

Size: 1×96 wells
Sensitivity: Cut-off control
Incubation Time: 1.5 Hours
Sample Type: Serum
Species Sample: Human
Sample Size: 10 µL
Alternative Names: Serology, Coronavirus, COVID-19, Serological
RUO, CE Marked

Controls Included

Cross Reactivity

Panels were studied with a minimum of five confirmed disease state samples with this serology ELISA assay. No interference was observed for the following disease or infectious agents:

  • Anti-influenza A
  • Anti-influenza B
  • Hepatitis C (HCV)
  • Antinuclear Antibodies (ANA)
  • Respiratory Syncytial (RSV)

Assay Principle

The COVID-19 IgG ELISA Assay is designed, developed, and produced for the qualitative measurement of the COVID-19 IgG antibody in serum samples (serology). This serological assay utilizes the microplate based enzyme immunoassay technique.

Assay controls and samples are added to the microtiter wells of a microplate that was coated with the COVID-19 peptide antigen nucleocapsid protein. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase labeled COVID-19 IgG tracer antibody is added to each well. After an incubation period, an immunocomplex of ” COVID-19 polypeptide antigen – new coronavirus IgG antibody HRP labeled COVID-19 IgG tracer antibody ” is formed if there is coronavirus IgG antibody present in the tested materials. The unbound tracer antibody is removed by the subsequent washing step. HRP labeled tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the coronavirus IgG on the wall of the microtiter well is proportional to the amount of the coronavirus IgG antibody level in the tested materials.

Products Related to Coronavirus COVID-19 IgG ELISA Assay

Coronavirus COVID-19 IgM ELISA

COVID-19 Information

Additional Information

Assay Background

2019 novel coronavirus (COVID-19) is a single-stranded RNA coronavirus. Comparisons of the genetic sequences of is virus have shown similarities to SARS-CoV and bat coronaviruses. In humans, coronaviruses cause respiratory infections. Coronaviruses are composed of several proteins including the spike (S), envelope (E), membrane (M), and nucleocapsid (N). Results suggest that the spike protein retains sufficient affinity to the Angiotensin converting enzyme 2 (ACE2) receptor to use it as a mechanism of cell entry. Human to human transmission of coronaviruses is primarily thought to occur among close contacts via respiratory droplets generated by sneezing and coughing. IgG is the most abundantly found immunoglobulin to be produced in response to an antigen and will be maintained in the body after initial exposure for long term response.




The following papers utilize the Novel Coronavirus COVID-19 IgG or IgM ELISA Kits:

Lombardi, F., Belmonti, S., Ricci, R., et al. No evidence of SARS-CoV-2 circulation in HIV-infected patients between December 2019 and February 2020 in Rome, Italy. Clinical Microbiology and Infection. (2020)

Gudbjartsson, D. F., Norddahl, G. L., Melsted, P., Gunnarsdottir, K., Holm, H., Eythorsson, E., . . . Stefansson, K. (2020). Humoral Immune Response to SARS-CoV-2 in Iceland. New England Journal of Medicine. doi:10.1056/nejmoa2026116

Ahn JY, Sohn Y, Lee SH, et al. Use of Convalescent Plasma Therapy in Two COVID-19 Patients with Acute Respiratory Distress Syndrome in Korea. J Korean Med Sci. 2020;35(14):e149. Published 2020 Apr 13. doi:10.3346/jkms.2020.35.e149

Ambroise Pare Hospital, Evaluation report of the EDI test at Ambroise Paré Hospital. Assistance Publique Hospitaux de Parix. Published 2020 May 14.

Margo Egger, et al. Comparison of the Elecsys® Anti-SARS-CoV-2 immunoassay with the EDITM enzyme linked immunosorbent assays for the detection of SARS-CoV-2 antibodies in human plasma. Clinica Chimica Acta. Published 2020 May 28. doi/10.1016/j.cca.2020.05.049

Renata Varnaite, et al. Expansion of SARS-CoV-2-specific Antibody-secreting Cells and Generation of Neutralizing Antibodies in Hospitalized COVID-19 Patients. BioRXiv. Published 2020 May 29. doi/10.1101/2020.05.28.118729

Alexander Krüttgen et al. Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG. Journal of Clinical Virology. 2020;128:104394. Published 2020 Apr 22. doi:10.1016/2020.104394

Christian Bundschuh et al. Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma. Clinica Chimica Acta. Published 2020 June 8.