BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit

$750.00

The Eagle Biosciences BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit is intended for the quantitative determination of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in Plasma and Urine. The BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit

Size: 2 x 96 wells (1 plate for Adrenaline, 1 plate for Noradrenaline)
Sensitivity: Adrenaline (urine): 0.08 ng/ml, (plasma): 5 pg/ml, Noradrenaline (urine): 0.24 ng/ml, (plasma): 16 pg/ml
Dynamic Range: Adrenaline: 0.5 – 250 ng/ml, Noradrenaline: 1.5 – 500 ng/ml
Incubation Time:  Overnight
Sample Type:  Urine, Plasma
Sample Size: 20 µL (urine), 300 µl (plasma)

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika


Reference Values (Normal)

Urine:  Adrenaline = <20μg/day, Noradrenaline = < 90μg/day
Plasma: Adrenaline = < 100/pg/mL; Noradrenaline = < 600 pg/mL

Additional Information

Assay Background


The Eagle Biosciences BI-CAT Adrenaline & Noradrenaline ELISA  Assay Kit is intended for the quantitative determination of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in Plasma and Urine. The BI-CAT ® Adrenaline & Noradrenaline ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitters, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.

An increased production of catecholamines can be found with tumors of the chromaffine system (pheochromocytoma, neuroblastoma, ganglio­neuroma). An increased or decreased concentration of the catechol­amines can also be found with hypertension, degenerative cardiac diseases, schizophrenia and manic-depressive psychosis.  The BI-CAT ® Adrenaline & Noradrenaline ELISA assay kit provides materials for the quantitative measurement of adrenaline and noradrenaline in plasma and urine. Noradrenaline and adrenaline are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline and N-acyladrenaline and then converted enzymatically into N-acylnormeta­nephrine and N-acylmetanephrine.

Assay Principle


The competitive BI-CAT Adrenaline & Noradrenaline ELISA Assay Kit uses the microtiter plate format. Adrenaline and noradrenaline, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Typical Standard Curve



Manual

Product Manual


Publications

Citations


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