Serotonin Sensitive ELISA Kit
Serotonin Sensitive ELISA Kit manufactured in Germany as SKU EA630/96 by DLD Diagnostika
Size: 1×96 wells
Sensitivity: 0.39 pg/sample
Dynamic Range: 0.67 – 20 pg/sample
Incubation Time: Overnight
Sample Type: Biological Fluids
Sample Size: 20 µl
Alternative Names: 5-Hydroxytryptamine ELISA, 5-HT ELISA Assay Kit
For Research Use Only
The Serotonin Ultrasensitive ELISA provides materials for the quantitative measurement of derivated serotonin in low concentrated samples and for small sample volumes. The derivation is performed during the preparation of the samples. By using the acylation reagent the serotonin is quantitatively derivated into N-acylserotonin.
The competitive Serotonin Ultrasensitive ELISA kit uses the microtiter plate format. Derivated serotonin competes for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.