Calprotectin ELISA Assay Kit
$495.00
The Calprotectin ELISA Assay kit is intended for use in the quantitative determination of human calprotectin (neutrophil cytoplasmic protein S100A8/A9, MRP 8/14) levels in stool samples via Enzyme-linked immunosorbent assay. The Eagle Biosciences Calprotectin ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
Calprotectin ELISA Assay Kit
Calprotectin ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 5 ng/ml
Dynamic Range: 69.5 – 892ng/ml , 156 – 5560 ng/ml
Incubation Time: 2 hours
Sample Type: Stool
Sample Size:50mg
Alternative Names: MRP 8/14 ELISA
For Research Use Only
Controls Included
Note:
Calprotectin ng/mL X 0.36 = Calprotectin μg/g
Calprotectin μg/g X 2.78 = Calprotectin ng/mL
Assay Principle
This Calprotectin ELISA Kit is designed, developed and produced for the quantitative measurement of human calprotectin in stool samples. The Calprotectin ELISA utilizes the two-site “sandwich” technique with two selected antibodies that bind to different epitopes of human calprotectin.
Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to calprotectin. After a short incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human calprotectin specific monoclonal antibody is added to each well. After the second incubation period, a “sandwich” of solid-phase antibody – human calprotectin – HRP conjugated monoclonal antibody” is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance versus the respective human calprotectin concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of fecal human calprotectin in test samples is determined directly from this standard curve of the Calprotectin ELISA Assay.
Products Related to Calprotectin ELISA Assay Kit
NGAL (Stool) ELISA Assay Kit
S100A8/A9 (MRP8/14, Calprotectin, Mouse/Rat) ELISA Kit
Stool Sample Collection Kit
Related News
EagleBio’s Calprotectin ELISA Used in a Recent Study
North Carolina Study Utilizes An EagleBio Calprotectin ELISA
EagleBio’s Calprotectin ELISA Used in Recent NYU Study
Documents
Product Documents
Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.
Publications
Citations
Chen, K., Jin, S., Chen, H.,et al. Dose effect of bovine lactoferrin fortification on diarrhea and respiratory tract infections in weaned infants with anemia: A randomized, controlled trial. Nutrition. (2021)90:111288
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Doshi, H., Pandya, S., Codipilly, C.N., Schanler, R.J. Does Human Milk Fortifier Affect Intestinal Inflammation in Preterm Infants? Breastfeeding Medicine (2020) https://doi.org/10.1089/bfm.2020.0205
Asaro, J.A., Khan, Z., Brewer, M., et al. Relationship Between Milk Fat Globule-Epidermal Growth Factor 8 and Intestinal Cytokines in Infants Born Preterm. Journal of Pediatrics (2020) https://doi.org/10.1016/j.jpeds.2020.11.014
Haxaire, Coline, et al. “Blood-Induced Bone Loss in Murine Hemophilic Arthropathy Is Prevented by Blocking the iRhom2/ADAM17/TNFα Pathway.” Blood, 18 May 2018, doi:10.1182/blood-2017-12-820571.
Nicholson, Maribeth R., Jonathan D. Crews, Jeffrey R. Starke, Zhi-Dong Jiang, Herbert Dupont, and Kathryn Edwards. “Recurrent Clostridium difficile Infection in Children.” The Pediatric Infectious Disease Journal36.4 (2017): 379-83. Web.
Becker-Dreps, Sylvia, Samuel Vilchez, Filemon Bucardo, Erica Twitchell, Wan Suk Choi, Michael G. Hudgens, Johann Perez, and Lijuan Yuan. “The Association Between Fecal Biomarkers of Environmental Enteropathy and Rotavirus Vaccine Response in Nicaraguan Infants.” The Pediatric Infectious Disease Journal36.4 (2017): 412-16. Web.
Zackular, JP;Moore, JL;Jordan, AT;Juttukonda, LJ;Noto, MJ;Nicholson, MR;Crews, JD;Semler, MW;Zhang, Y;Ware, LB;Washington, MK;Chazin, WJ;Caprioli, RM;Skaar, EP; Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection. Nat. Med. 2016. doi: 10.1038/nm.4174
Shaw, KA;Bertha, M;Hofmekler, T;Chopra, P;Vatanen, T;Srivatsa, A;Prince, J;Kumar, A;Sauer, C;Zwick, ME;Satten, GA;Kostic, AD;Mulle, JG;Xavier, RJ;Kugathasan, S; Dysbiosis, inflammation, and response to treatment: a longitudinal study of pediatric subjects with newly diagnosed inflammatory bowel disease. Genome Med 2016 Vol.8:1, pg. 75. DOI: 10.1186/s13073-016-0331-y
Prata, Md;A, H;DT, B;R, P;AAM, L;RL, G; Comparisons between myeloperoxidase, lactoferrin, calprotectin and lipocalin-2, as fecal biomarkers of intestinal inflammation in malnourished children. 2016 J Transl Sci: ISSN: 2059-268X; Vol. 2, Iss. 2, pg.75.
Hall, A. B., Yassour, M., Sauk, J., Garner, A., Jiang, X., Arthur, T., . . . Huttenhower, C. (2017). A novel Ruminococcus gnavus clade enriched in inflammatory bowel disease patients. Genome Medicine, 9(1), 103-103. doi:10.1186/s13073-017-0490-5