Homoarginine ELISA Assay Kit

$1,830.00

The Eagle Biosciences Homoarginine ELISA Assay Kit is intended to be used for the in vitro quantitative determination of homoarginine in serum, plasma, or cell culture samples. The Homoarginine ELISA Assay Kit is for reseach use only and not for use in diagnostic or therapeutic procedures.

SKU: HOM39-K01 Categories: , , Tags: , ,

Homoarginine ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.05 µmol/L
Dynamic Range: 0.03 – 7.0 µmol/l
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 20 µL

Controls Included

Product Developed and Manufactured in Germany by DLD Diagnostika 


Additional Information

Assay Background


Homoarginine is a non-essential cationic amino acid, which is formed from lysine.  In vitro and in vivo, homoarginine shows characteristics similar to arginine. Epidemiological investigations in two large independent cohorts, namely the German diabetes dialysis (4D) study and the Ludwigshafen Risk and Cardiovascular Health (LURIC) study have identified homoarginine as useful predictor of cardiovascular events and mortality.

Beyond potential cardiovascular indications, homoarginine concentrations are known to be directly correlated with kidney function and are known to be significantly associated with the progression of chronic kidney disease (CKD). Low homoarginine concentrations may be an early indicator of kidney failure and may be a potential target for the prevention of disease progression which needs further investigation. Furthermore homoarginine could be a useful marker for the monitoring of hemodialysis patients.

Eagle Biosciences offers a competitive Homoarginine ELISA Assay Kit using the microtiter plate format. The correlation of the ELISA method to LC-MS is exceptionally good. In addition, no interferences with any therapeutic drugs are observed. The Homoarginine ELISA Assay Kit method allows the measurement of large series of samples.

Assay Principle


The competitive Homoarginine ELISA Assay Kit uses the microtiter plate format. Homoarginine is bound to the solid phase of the microtiter plate. Homoarginine in the samples is acylated and competes with solid phase bound Homoarginine for a fixed number of rabbit anti-Homoarginine antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase Homoarginine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Homoarginine is inversely proportional to the Homoarginine concentration of the sample.

Typical Standard Curve


Manual

Product Manual


Publications

Citations


Cordts 2015 Vergleich HA mittels LCMS und ELISA