Author: Eagle Biosciences

Intracerebral hemorrhage (ICH) is caused by bleeding within the brain. Very few circulating biomarkers are known to be associated with the risk of ICH. Fibroblast growth factor 23 is a bone-derived protein hormone associated with mortality in patients with heart failure. A recent nested case–control study showed that Fibroblast growth factor 23 is associated with risk of intracerebral hemorrhage: Fibroblast growth factor 23 is associated with risk of intracerebral hemorrhage. Svensson EH, Söderholm M. Eur J Neurol. 2022 Jan;29(1):114-120. doi: 10.1111/ene.15060. PMID: 34379844.

Abstract

Background and purpose: Fibroblast growth factor 23 is an osteogenic hormone associated with chronic kidney disease and is an emerging risk factor for several cardiovascular diseases. The association of Fibroblast growth factor 23 (FGF23) with stroke is unclear. The aim of this study was to investigate the association of FGF23 with incident intracerebral hemorrhage (ICH).

Methods: This was a nested case-control study of 220 ICH cases and 244 age- and sex-matched controls from the population-based Malmö Diet and Cancer Study (n = 28,449). Incident ICH cases were ascertained using national registers and classified by bleeding location. Logistic regression was used to study the association of plasma levels of FGF23 with incident ICH, adjusting for potential ICH risk factors. Subgroup analyses were performed for lobar and non-lobar ICH, fatal ICH, ICH with large volume and ICH with poor functional outcome, respectively.

Results: Higher FGF23 levels at baseline were significantly associated with incident ICH. After multivariable adjustment, the odds ratio for the association with all ICH was 1.84 (95% confidence interval [CI] 1.25-2.71, p = 0.002) per doubling of FGF23 concentration. For lobar and non-lobar ICH, odds ratios were 1.73 (95% CI 1.04-2.87, p = 0.035) and 2.13 (95% CI 1.32-3.45, p = 0.002), respectively. FGF23 was also significantly associated with fatal ICH, ICH with large volume and ICH with poor functional outcome.

Conclusions: Higher FGF23 was associated with incident ICH in this nested case-control study. Further studies are required to explore whether the association is causal.

Eagle Biosciences’ FGF23 kits listed below:

FGF23 C-Terminal ELISA Assay Kit
MedFrontier Intact FGF23 Assay

If you have any questions about this items or any of our other offerings, contact us here.

Eagle Biosciences is proud to highlight our extensive range of Therapeutic Drug Monitoring (TDM) Assays!

Why use TDM Assays?

TDM Assays for biosimilars and biologics are developed primarily for the studies and development of drugs and vaccines in specific therapeutics and therapeutic pathways. They are utilized by pharmaceutical and biopharmaceutical companies, as well as universities and other researchers to aide in a wide variety of research fields.

About TDM Assays

It has long been acknowledged that data on different drug and target species (e.g., free vs total levels of drug target as two possible biomarkers) may satisfy different needs. Depending on the information required for decision-making, the data required and hence the selection of assay (free, total or both) may differ at each phase of drug development.

Free drug levels reflect the availability of the active drug, while the total or bound drug complex is of importance when checking for efficacy or dynamic interactions of the drug, and for other PK/PD assessments. Thus, it is important to understand the information needed at different stages of drug development.

We offer a wide range of products to measure free and partially bound drug, as well as total drug (free, bound, partially bound), and the bound drug complex.

And we’ll be expanding our extensive line in the future!

If you have any questions about our TDM Assay Kits or our other offerings, contact us here.

Understanding the Microbiome with Pioneering Research at Genetic Analysis

Anita Jusnes and Christin Casén of Genetic Analysis AS were recently featured on Dr. Ruscio Radio! They discuss with Dr. Ruscio the GA-Map Dysbiosis Test Lx, the only research dysbiosis index for the gut. The conversation covers topics including but not limited to, how the test came to be, the methodology behind the results, how the test has been validated, and how it can be used to distinguish healthy controls from other samples.

Find the full episode and transcript here.

About the GA-map Dysbiosis Test

The human microbiome market is accelerating both in terms of evidence-based research and pharma products launched. The market’s need for a validated microbiome test is growing. Genetic Analysis AS is at the forefront of this development with the GA-map Dysbiosis Test. The unique, GA-map® Dysbiosis Test, detects and characterizes imbalance in the gut microbiota (dysbiosis) in human fecal samples. All pre-processing of the data is done by the GA-map® Dysbiosis Test analyze software. Reports are automatically generated on the dysbiosis index (DI) and the bacteria abundance. The DI can be customized to your needs. Based on a deviation from a normal reference population, a measure of bacteria abundances and deviations to the references are calculated. The results are presented in an easy to interpret report form, containing the 48 preselected bacteria markers.

Additionally, Genetic Analysis has developed a fecal COVID-19 test that is performed on the same GA-map platform and is an important research tool in the analysis of an individual’s COVID-19 status.

If you have any questions about the GA-map Dysbiosis Test Lx or any of our other offerings, contact us here.

A recent study shows a strong association between the development of hyperphosphatemia in dogs with chronic kidney disease and increased serum levels of fibroblastic growth factor-23 (FGF-23)

Study:
Miyakawa H, Hsu H-H, Ogawa M, Akabane R, Miyagawa Y, Takemura N. Association between serum fibroblast growth factor-23 concentration and development of hyperphosphatemia in normophosphatemic dogs with chronic kidney disease. J Vet Intern Med. 2021;35(5):2296-2305. Full Text.

Background

Chronic kidney disease (CKD) is a common, irreversible, and progressive disease in dogs. In patients with CKD, mineral metabolism disorders such as hyperphosphatemia, renal hyperparathyroidism, and decreased calcitriol synthesis also occur because of impaired renal function. These disorders are termed CKD—mineral and bone disorder (MBD). Hyperphosphatemia (abnormally high serum phosphate levels) is known to be a prognostic factor for shorter survival time in dogs with CKD.

The role of FGF-23 in CKD

Fibroblast growth factor-23 is released from osteocytes in response to increased serum phosphorus and calcitriol concentrations and promotes phosphate excretion into the urine by downregulation of the sodium-phosphate co-transporter in renal proximal tubular cells and inhibition of calcitriol synthesis. Fibroblast growth factor-23 acts by binding to the FGF receptor-α-klotho complex.13 In humans, cats, and dogs with CKD, circulating FGF-23 concentrations have been shown to increase in the advanced CKD stages. Increased FGF-23 concentration in CKD patients is associated with various mechanisms, such as a decreased clearance of FGF-23 because of decreasing glomerular filtration rate (GFR), compensation for the accumulation of phosphate in the body, and compensation for decreased klotho protein concentrations. Blood FGF-23 concentrations in dogs with CKD have been shown to become increased earlier than serum phosphorous concentrations therefore, FGF-23 has been noted as an early marker of CKD-MBD.

Researchers hypothesized that FGF-23 predicts development of hyperphosphatemia in normophosphatemic dogs with CKD. If correct, FGF-23 may be a useful marker of when to initiate a phosphate-restricted diet to prevent the development of hyperphosphatemia.

About the MedFrontier Intact FGF23 Assay

• low sample volume required – only 20ul
• ONLY assay that measures the full-length active form (intact form) of FGF23
• superior dynamic range of 10-3000 pg/uL
• CLEIA technology

The MedFrontier Intact FGF23 Assay was developed and manufactured by Minaris Medical.

IF you have any questions about this product or any of our other products, contact us here.

One of our supplier’s was recently featured in an editorial by Select Science! Svar Life Sciences has been working closely with Dr. Erik Tillman at Akero Therapeutics. Tillman and his team are developing a multi-modal drug that could help treat the drivers of non-alcoholic steatohepatitis (NASH), a severe form of non-alcoholic related fatty liver disease (NAFLD) that has increased risk for cardiovascular and liver related morbidity and mortality. Him and his team have been working with Svar to create custom iLite assay-ready cells for the cell-based assays that have become a key role in the work Tillman and his teammates perform daily.

“The assay-ready cells have been much more robust and the results have been more reproducible than other cell-based assays that we had tried to use or develop in the past,” says Tillman. “Using the assay-ready cells from Svar, we understand how the cells have been made and characterized, and we are confident in how the cells are going to perform from one vial to the next, and from one lot to the next. Svar’s expertise in the engineering of the cell system and our results thus far have given us the confidence to broaden our use of the assay-ready cells.”

Click here to read the full editorial about Dr. Tillman’s research and the role of Svar’s iLite assay-ready cells.

If you have any questions about Svar’s iLite Assay Ready Cells or our other offerings contact us here.