NSE ELISA Assay Kit
NSE ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 1.2 ng/mL
Dynamic Range: 5.0 – 178 ng/ml
Incubation Time: 1.0 hours
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Human Neuron Specific Enolase
For Research Use Only
Expected Normal Values:
One hundred seventy two normal adult sera were measured with this human Neuron Specific Enolase (NSE) ELISA. The normal range was found to be less than 15 ng/mL. It is highly recommend that each laboratory should establish its own normal cut off level.
The NSE ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Neuron Specific Enolase in serum sample. The assay utilizes the two-site “sandwich” technique with two selected monoclonal antibodies that bind to different epitopes of the γ-subunit of the enzyme.
Assay standards, controls and samples are added directly to wells of microplate that is coated with a streptavidin. Subsequently, a mixture of a biotinylated NSE specific monoclonal antibody and a horseradish peroxidase (HRP) labeled NSE specific monoclonal antibody is added to each microtiter well. After the first incubation a “sandwich” immunocomplex of “streptavidin-biotin-monoclonal antibody – human NSE – monoclonal antibody-HRP” is formed. The unbound monoclonal antibodies are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the NSE on the wall of the microtiter well is directly proportional to the amount of NSE in the sample. A standard curve is generated by plotting the absorbance versus the respective human NSE concentration for each standard on point-to-point, cubical scales or 4 parameter curve fit. The concentration of human NSE in test samples is determined directly from this standard curve.