Pepsinogen II ELISA Kit
Pepsinogen II ELISA Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 6.3 – 100 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum
Sample Size: 50 µL
Alternative Names: Pepsinogen 2
For Research Use Only
The Pepsinogen II ELISA Kit is designed, developed and produced for the quantitative measurement of human pepsinogen II level in serum sample. The assay utilizes the two-site “sandwich” technique with two selected monoclonal antibodies that bind to different epitopes of human pepsinogen II without any cross-reaction to human pepsinogen I.
Assay standards, controls and patient serum samples containing human pepsinogen II is added directly to microtiter wells of microplate that was coated with a streptavidin. Simultaneously, a biotinylated antibody and a horseradish peroxidase conjugated antibody is added to each well. After the first incubation period, on the wall of microtiter well captures the biotinylated antibody as well as an immuno complex in the form of “streptavidin – biotin-antibody – pepsinogen II – HRP-antibody”. Unbound proteins as well as unbound HRP conjugated antibody in each microtiter well are removed in the subsequent washing step. The well is incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the pepsinogen II on the wall of the microtiter well is directly proportional to the amount of pepsinogen II in the sample. A standard curve is generated by plotting the absorbance versus the respective human pepsinogen II concentration for each standard on Point-to-Point, CubicSpline or 4-Parameter plot. The concentration of human pepsinogen II in test samples is determined directly from this standard curve.