Serotonin ELISA Assay Kit

$465.00

The Serotonin ELISA Assay Kit is intended for the quantitative determination of serotonin in Serum, Plasma, and Urine. The Serotonin ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

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Serotonin ELISA Assay

Serotonin ELISA Assay Kit Developed and Manufactured in Germany by DLD Diagnostika EA602/96

Size:  1×96 wells
Sensitivity:  4.7 ng/ml for serum and urine; 2.6 ng/mL for plasma
Dynamic Range:  15 – 2500 ng/mL
Incubation Time:  2.5 hours
Sample Type:  Serum, Plasma, and Urine
Sample Size: 20 µl
Alternative Names: 5-HT, 5-Hydroxytryptamine
For Research Use Only

Controls Included


Normal Ranges for Serotonin ELISA
Plasma (platelet-free):
< 10 ng/ml
Serum:
Female 80 – 450 ng/ml
Male 40 – 400 ng/ml
Urine:
50 – 250 μg/day
It is suggested that each laboratory establish its own normal ranges

Conversion
Serotonin: 1 ng/ml = 5.675 nmol/l


Assay Background for Serotonin ELISA

Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain.  Central-serotonergic neurons influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.
The Serotonin ELISA provides materials for the quantitative measurement of derivated serotonin (5-Hydroxytryptamine, 5-HT) in serum, plasma and urine. The derivation is performed during the preparation of the samples. By using the acylation reagent the serotonin is quantitatively derivated into N-acylserotonin.


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Additional Information

Assay Principle


The competitive Serotonin ELISA Assay kit uses the microtiter plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.

Typical Standard Curve


Serotonin ELISA Assay Kit

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