Mouse Rat GLP-1 Active (7-36) ELISA
Mouse Rat GLP-1 Active (7-36) ELISA Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.1 pM
Dynamic Range: 0.85 – 146 pM
Incubation Time: Overnight
Sample Type: Plasma*
Sample Size: 20 µL
Alternative Names: Mouse / Rat Glucagon-like-peptide-1 Active ELISA, Rodent GLP-1 Active ELISA
For Research Use Only
GLP-1(7-36) pg/ml = GLP-1 (7-36) pM x 3.298
*The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc.
The Mouse / Rat Active GLP-1 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in rodent plasma where there is usually have limited amount of sample available for analysis. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 (7-36) specific antibodies.
The Mouse Rat GLP-1 Active (7-36) ELISA has standards, controls and test samples that are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample.