B2-Microglobulin ELISA Assay Kit
The B2 Microglobulin ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.1 mg/L
Dynamic Range: 0.2–10 mg/L
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 20 μL
Specificity for Beta 2 Microglobulin ELISA Assay
The following compounds were tested for cross-reactivity with the Direct β2-M ELISA kit with β2-M cross-reacting at 100%.
Human IgG: <0.00001
Expected Normal Values for B2 Microglobulin Assay
As for all clinical assays each laboratory should collect data and establish their own range of expected normal values.
Males Age 24-70: 1.15-3.85 mg/L
Females Age 19-45: 0.73-3.56 mg/L
Postmenopausal Females: 1.28-2.34 mg/L
Young Males and Females, Age 3-18: 0.89-1.36 mg/L
This assay is a direct system. No specimen pretreatment is necessary.
The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of β2 -M in the sample. A set of standards is used to plot a standard curve from which the amount of β2-M in patient samples and controls can be directly read.