Endocrinology ELISA Assay

B2-Microglobulin ELISA Assay Kit

$270.00

The Eagle Biosciences B2-Microglobulin ELISA Assay Kit is intended for the direct quantitative determination of β2 -Microglobulin by an enzyme immunoassay in human serum.

SKU: B3M31-K01 Categories: , ,

B2-Microglobulin ELISA Assay Kit

The B2 Microglobulin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 mg/L
Dynamic Range: 0.2–10 mg/L
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 20 μL
Controls Included

Specificity for Beta 2 Microglobulin ELISA Assay
The following compounds were tested for cross-reactivity with the Direct β2-M ELISA kit with β2-M cross-reacting at 100%.
B2-M: 100%
Human IgG: <0.00001 Expected Normal Values for B2 Microglobulin Assay
As for all clinical assays each laboratory should collect data and establish their own range of expected normal values.
Males Age 24-70: 1.15-3.85 mg/L
Females Age 19-45: 0.73-3.56 mg/L
Postmenopausal Females: 1.28-2.34 mg/L
Young Males and Females, Age 3-18: 0.89-1.36 mg/L
SPECIMEN PRETREATMENT
This assay is a direct system. No specimen pretreatment is necessary.

Assay Background for B2-Microglobulin

Beta 2-Microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β 2 -M is continuously secreted into the circulatory system and therefore maintains a balanced serum level.
Clinical Trends:
• Decreases in the glomerular filtration rate gives rise to increases in the level of β2-M.
• β2 -M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis.
• Patients with acquired immune deficiency syndrome (AIDS) show an increased level of β2-M.
β2 -M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2 -M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and finally for young people = 1.13 mg/L.

Related Products for Beta 2 Microglobulin ELISA

Cortisol ELISA Assay Kit
DHEA ELISA Assay Kit
DHEA-S ELISA Assay Kit

Additional Information

Assay Background

β2-Microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β 2 -M is continuously secreted into the circulatory system and therefore maintains a balanced serum level.
Clinical Trends:
• Decreases in the glomerular filtration rate gives rise to increases in the level of β2-M.
• β2 -M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis.
• Patients with acquired immune deficiency syndrome (AIDS) show an increased level of β2-M.
β2 -M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2 -M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and finally for young people = 1.13 mg/L.

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of β2 -M in the sample. A set of standards is used to plot a standard curve from which the amount of β2-M in patient samples and controls can be directly read.

Typical Standard Curve

Documents

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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