B2-Microglobulin ELISA Assay Kit

$250.00

The Eagle Biosciences B2-Microglobulin ELISA Assay Kit is intended for the direct quantitative determination of β2 -Microglobulin by an enzyme immunoassay in human serum.

SKU: B3M31-K01 Categories: , ,

B2-Microglobulin ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 mg/L
Dynamic Range: 0.2–10 mg/L
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 20 μL

Controls Included

Additional Information

Assay Background


β2-Microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β 2 -M is continuously secreted into the circulatory system and therefore maintains a balanced serum level.
Clinical Trends:
• Decreases in the glomerular filtration rate gives rise to increases in the level of β2-M.
• β2 -M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis.
• Patients with acquired immune deficiency syndrome (AIDS) show an increased level of β2-M.
β2 -M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2 -M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and finally for young people = 1.13 mg/L.

Assay Principle


The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of β2 -M in the sample. A set of standards is used to plot a standard curve from which the amount of β2-M in patient samples and controls can be directly read.

Typical Standard Curve


Manual

Product Manual