B2-Microglobulin ELISA Assay Kit
The B2 Microglobulin ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.1 mg/L
Dynamic Range: 0.2–10 mg/L
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 20 μL
Specificity for Beta 2 Microglobulin ELISA Assay
The following compounds were tested for cross-reactivity with the Direct β2-M ELISA kit with β2-M cross-reacting at 100%.
Human IgG: <0.00001 Expected Normal Values for B2 Microglobulin Assay
As for all clinical assays each laboratory should collect data and establish their own range of expected normal values.
Males Age 24-70: 1.15-3.85 mg/L
Females Age 19-45: 0.73-3.56 mg/L
Postmenopausal Females: 1.28-2.34 mg/L
Young Males and Females, Age 3-18: 0.89-1.36 mg/L
This assay is a direct system. No specimen pretreatment is necessary.
Assay Background for B2-Microglobulin
Beta 2-Microglobulin (β2-M) is a single polypeptide chain containing 100 amino acids and is found on the surface of nucleated cells. β 2 -M is continuously secreted into the circulatory system and therefore maintains a balanced serum level.
• Decreases in the glomerular filtration rate gives rise to increases in the level of β2-M.
• β2 -M is a helpful marker in the diagnosis of kidney disease and active rheumatoid arthritis.
• Patients with acquired immune deficiency syndrome (AIDS) show an increased level of β2-M.
β2 -M has a low concentration in serum. We have found in a normal unselected population that in serum the highest level for β2 -M is 3.8 mg/L. The total number of serum samples tested was 92, showing little differences in the normal level in males, pre- and postmenopausal females. The average for male samples = 1.582 mg/L, for premenopausal female = 1.457 mg/L, for postmenopausal female = 1.608 mg/L and finally for young people = 1.13 mg/L.