DHEA ELISA Assay Kit

$240.00

The Eagle Biosciences DHEA ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Dehydroepiandrosterone (DHEA) concentration in human serum or plasma. The DHEA ELISA Assay Kit is intended for research use only and is not intended for diagnostic procedures.

SKU: DCM124 Categories: , ,

DHEA ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.10 ng/mL
Dynamic Range: 0.5 – 30 ng/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Dehydroepiandrosterone(DHEA) is a C19 steroid secreted by the adrenal cortex and in smaller quantities by the gonads. DHEA is a precursor in testosterone and various estrogens biosynthesis.

The physiologic role of DHEA is not-well defined, because of a lot of ascertained in vivo and in vitro effects, for example the prevention and regression of colon tumor, spontaneous and inducted, in rodents.

Some studies showed a decrease in the DHEA production in women with risk for the breast cancer. In animal models DHEA showed a role in the therapeutic action against diabetes, obesity and cardiovascular pathologies, and unclear roles in immunology, in lipid metabolism (also cholesterol) and in the nervous system.

Seric levels of DHEA are relatively high in fetal and neonatal age, decrease during childhood and increase again during puberty until 30 years. No DHEA variations have been observed during pregnancy or menstrual cycle. Seric levels of DHEA are 100-1000 times lower than the levels of DHEA sulphate (DHEA-S). The measurement of seric levels of DHEA is a marker for adrenal androgen synthesis. Abnormal low levels of DHEA can be observed in the hypoadrenalism, while elevated levels can be observed in various pathologies such as the surrenalic adenomas, 21-hydroxylase and 3b-hydroxysteroid dehydrogenase deficiency and in some cases of female hirsutism.

Assay Principle

In the DHEA ELISA Assay Kit, the essential reagents required for this immunoenzymatic assay are a biotinylated anti DHEA antibody,  the DHEA antigen conjugated with the enzyme HRP (horseradish peroxidase), the DHEA antigen present in the sample, and a microplate coated with Streptavidin (highly specific for Biotin). The quantity of native antigen is unknown, while the antibody and the antigen linked to HRP are in excess.
In the first part of the DHEA ELISA Assay Kit these three components are mixed together; a competitive reaction for the anti DHEA antibody between the native antigen and the antigen linked to HRP develops.

Simultaneously to their formation, the complexes are fixed to the microplate wells through the interaction between the Streptavidin and the biotinylated antibody.

The DHEA ELISA Assay Kit reagents in excess that have not reacted are eliminated in the washing steps.

In the last part of the assay, the enzyme HRP linked in the wells reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the native antigen DHEA in the sample. DHEA concentration in the sample is finally calculated through a calibration curve.

Manual

Product Manual


Publications

Publications

Cai, Hualing et al. (2018). Pregnenolone-progesterone-allopregnanolone pathway as a potential therapeutic target in first-episode antipsychotic-naïve patient with schizophrenia. Psychoneuroendocrinology. 90:43-51.