Chorionic Gonadotropin (hCG) ELISA Assay Kit

$190.00

The Eagle Biosciences Chorionic Gonadotropin (hCG) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Chorionic Gonadotropin (hCG) in human serum by an enzyme immunoassay. The Chorionic Gonadotropin (hCG) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: HCG31-K01 Categories: , ,

Chorionic Gonadotropin (hCG) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.7 IU/L
Dynamic Range: 2.5–500 IU/L
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 25 µL

Controls Included

Additional Information

Assay Background


Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by the placenta. During normal pregnancy the level of this hormone in serum and urine gradually increases up to about the eighth week of pregnancy. hCG has two subunits, namely α and β. The α subunit is similar to the α subunit of the anterior pituitary gland glycoprotein hormones, namely, LH, TSH and FSH. However, the β subunits of these hormones are different. Therefore, this uniqueness distinguishes one hormone from the other, hence specificity. In raising this monoclonal antibody, the immunogen used was βhCG, which helps to make the assay system very specific and sensitive. In normal pregnancy the increase in hCG starts at about the 5th day after conception and continues to rise until it reaches a maximum at about the eighth week. In some pathological conditions the level of hCG in serum and/or urine is increased. It is a well known fact that hCG is also a tumour marker which is very important in the diagnosis of choriocarcinoma. In the case of hydatiform mole, hCG is also elevated. In about 50% of patients with testicular teratomas the level of hCG is elevated. It is also relevant to note that hCG is a good indicator, in order to follow the response to treatment.

Assay Principle


The principle of the following enzyme immunoassay test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for hCG is immobilized onto the microplate and another monoclonal antibody specific for a different region of hCG is conjugated to horse radish peroxidase (HRP). hCG from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of hCG in the sample. A set of standards is used to plot a standard curve from which the amount of hCG in patient samples and controls can be directly read.
Please note that a two-step procedure is included and is to be used for assaying serum from pregnant women.

Typical Standard Curve


Manual

Product Manual