Cortisol ELISA Assay Kit


Eagle Biosciences Cortisol ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Cortisol concentration in human serum or plasma. Cortisol ELISA Assay Kit is intended for research use only and is not intended for diagnostic procedures.

SKU: DCM001 Categories: , ,

Cortisol ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 2.44 ng/mL
Dynamic Range: 10 – 500 ng/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 20 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Cortisol is a steroid hormone released from the adrenal cortex in response to a hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid-binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors.
The amount of cortisol present in the serum undergoes diurnal variation, with the highest levels present in the early morning, and lower levels in the evening, several hours after the onset of sleep. Highest levels are at about 6-8 a.m. and lowest levels are at about midnight. These normal endogenous functions are the basis for the physiological consequences of chronic stress -prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune/inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.

Assay Principle

In the Cortisol ELISA Assay Kit, the Cortisol (antigen) in the sample competes with the antigenic Cortisol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti- Cortisol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing.
Then, the enzyme HRP in the bound-fraction reacts with the Substrate(H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the Cortisol concentration of in the sample. Cortisol concentration in the sample is calculated through a calibration curve.


Product Manual


Recent Citations

Khan, MDM;, Assessment of the role of elderberry extract in Nile tilapia, Oreochromis niloticus, as a stress reducing and immune stimulating nutraceutical in-vitro and in-vivo (Dissertation, Purdue University, 2016).