Cortisol ELISA Assay Kit
The Cortisol ELISA Assay Kit is for Research Use Only
Size: 1×96 wells
Sensitivity: 0.4 μg/dL
Dynamic Range: 0.5–60 μg/dL
Incubation Time: 65 minutes
Sample Type: Serum
Sample Size: 20 µL
Assay Background for Cortisol ELISA
Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1–2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva, salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at-10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Expected Normal Range
As for all clinical assays each laboratory should collect data and establish their own range of expected normal values.
Males and Females, AM: 3.95-27.23 μg/dL
Males and Females, PM: 1.45-10.41 μg/dL