Cortisol ELISA Assay Kit


The Cortisol ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Cortisol in human serum by an enzyme immunoassay. The Cortisol ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: COR31-K01 Categories: , ,

Cortisol ELISA Assay Kit

The Cortisol ELISA Assay Kit is for Research Use Only

Size: 1×96 wells
Sensitivity: 0.4 μg/dL
Dynamic Range: 0.5–60 μg/dL
Incubation Time: 65 minutes
Sample Type: Serum
Sample Size: 20 µL
Controls Included

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of cortisol in the sample. A set of standards is used to plot a standard curve from which the amount of cortisol in patient samples and controls can be directly read.

Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at-10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Expected Normal Range
As for all clinical assays each laboratory should collect data and establish their own range of expected normal values.
Males and Females, AM: 3.95-27.23 μg/dL
Males and Females, PM: 1.45-10.41 μg/dL

Related Products

Cortisol LIA Assay Kit
Urinary Cortisol ELISA Kit
Cortisol Saliva Assay Kit

Additional Information

Assay Background for Cortisol ELISA

Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison’s and Cushing’s diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1–2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva, salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.

Typical Standard Curve

Cortisol ELISA Assay Kit

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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