DHEA-S ELISA Assay Kit

$230.00

The DHEA-S ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Dehydro-epiandrosterone sulfate (DHEA-S) concentration in human serum or plasma. DHEA-S ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM005 Categories: , ,

DHEA-S ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.03 mg/mL
Dynamic Range: 0.1 – 10 μg/mL
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 20 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Dehydroepiandrosterone sulfate (DHEA-S), is a natural steroid hormone found atop of the kidneys in the human body. DHEA-S derived from enzymatic conversion of DHEA in adrenal and extradrenal tissues. DHEA-S is also produced in the gonads, adipose tissue and the brain. It is the most abundant hormone in the human body and it is precursor of all sex steroids.  As most DHEA-S is produced by the zona reticularis of the adrenal, it is argued that there is a role in the immune and stress response. DHEA-S may have more biologic roles. Its production in the brain suggests that is also has a role as a neurosteroid.

Measurement of serum DHEA-S is a useful marker of adrenal androgen synthesis. Abnormally low levels may occur in have been reported in hypoadrenalism, while elevated levels occur in several conditions, e.g. virilizing adrenal adenoma and carcinoma, 21-hydroxylase and 3β-hydroxysteroid dehydrogenase deficiencies and in some cases of female hirsutism. Women with polycystic ovary syndrome tend to have normal or mildly elevated levels of DHEAS. As very little DHEA-S is produced by the gonads, measurement of DHEA-S levels may aid in the localization of androgen source in virilizing conditions.

Assay Principle

In the DHEA-S ELISA Assay Kit, the (antigen) in the sample competes with the antigenic DHEA-S conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti DHEA-S coated on the microplate (solid phase). After the incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the DHEA-S concentration of in the sample. DHEA-S concentration in the sample is calculated through a calibration curve.

Manual

Product Manual