The DHEA-S ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human DHEAS levels in serum. The Dehydrospiandrosterone Sulfate (DHEAS) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: DHS31-K01 Categories: , ,


DHEA-S ELISA Assay Kit is for research use only

Size: 1×96 wells
Sensitivity: 0.005 μg/mL
Dynamic Range: 0.005–10 μg/mL
Incubation Time: 65 minutes
Sample Type: Serum
Sample Size: 25 µL
Controls Included

Approximately 0.1 mL of serum is required per duplicate determination in this DHEA-S Assay. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Specificity (Cross Reactivity)
The following compounds were tested for cross-reactivity with the DHEA-S ELISA Assay kit with DHEA-S cross-reacting at 100%:
DHEAS 100%
Androsterone 16.0%
Androstenedione 1.7%
Testosterone 0.9%
Progesterone 0.6%
DHT 0.6%
Cortisol 0.5%
The following steroids were tested but cross-reacted at less than 0.001% for this DHEA-S ELISA: 17β-Estradiol, Estrone, Estrone-Sulfate and Pregnenolone.

Assay Principle for Dehydrospiandrosterone Sulfate ELISA Assay

The DHEA-S ELISA Assay Kit follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHEAS in the sample. A set of standards is used to plot a standard curve from which the amount of DHEAS in patient samples and controls can be directly read.

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Additional Information

Assay Background

Dehydroepiandrosterone sulfate (DHEAS) is produced by the adrenals and gonads. As a result, the determination of the level of DHEA-S in serum is important in the evaluation of the functional state of these glands. DHEAS is a precursor of testosterone and estrone. Besides the adrenals in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle.
The principle production of testosterone in females is from conversion of other related androgens, especially DHEAS. An abnormal testosterone level in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with Elisa of DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.

Typical Standard Curve

Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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