PMN-Elastase Serum/Plasma ELISA Assay Kit
PMN-Elastase Serum/Plasma ELISA Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Dynamic Range: 0.37-10 ng/ml
Sensitivity: in plasma of a healthy person: 19–78 ng/ml, in serum of a healthy person: 186–1991 ng/ml
Incubation Time: 1 hour, 10-20 minutes
Sample Type: Serum, plasma,
Sample Size: 20 µl
For Research Use Only
PMN Elastase concentrations in plasma of a healthy person (n = 37): 19–78 ng/ml, -in serum of a healthy person (n = 52): average = 688 ng/ml (186–1991 ng/ml)
We recommend each laboratory to establish its own reference range.
In this PMN-Elastase Serum Plasma ELISA, PMN elastase in the sample is bound to polyclonal rabbit- anti-PMN elastase antibodies (in excess), which are immobilised on the surface of the microtiter wells (PLATE). To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody (AB) is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (α1-proteinase inhibitor = α1-antitrypsin). The quantification of the bound PMN elastase is carried out by adding an anti-mouse peroxidase- labeled conjugate (CONJ). Finally, the PMN elastase – antigen – antibody complex is incubated with the peroxidase substrate, tetramethylbenzidine (SUB). An acidic stop solution (STOP) is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the calibrators. PMN elastase, present in the patient samples, is determined directly from this curve. The combination of two specific antibodies in the PMN elastase ELISA drastically reduces the possibility of false results and offers a reliable diagnostic system to the user.