Author: Eagle Biosciences

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UPDATE: Calretinin ELISA Assay Used in a New Study!

by Eagle Biosciences

EagleBio’s Calretinin ELISA  Assay Kit was recently used in a study for the detection of malignant mesothelioma. This assay is a part of our Cancer Biomarker Kit line which is comprised of unique,versatile set of products for a variety of applications, research, and fields of study. Check out the product pages for full details on the kit or view the links below to find a new publication referencing our Calretinin ELISA Assay Kit.

Johnen, G., Gawrych, K., Raiko, I,. et al. “Calretinin as a blood-based biomarker for mesothelioma.” BMC Cancer. (2017) Vol. 17:386

“Concentrations of calretinin in plasma and serum samples were determined as described. In brief, a 1:1500 dilution of purified rabbit polyclonal anti-calretinin was used as capture antibody and a 1:5000 dilution of biotinylated polyclonal anti-calretinin as detection antibody. Samples (plasma or serum) were diluted 1:5 in Tris-buffered saline  supplemented with 5 mM CaCl2. A volume of 100 μl of a diluted sample was used for each determination. Calretinin concentrations were determined from a standard curve of human purified recombinant calretinin diluted between 10 and 0.08 ng/mL run in parallel on each plate. All determinations of calretinin were performed in the laboratory of the IPA.” Read More.

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Product Spotlight: Vedolizumab (Entyvio®) Antibodies ELISA Assay

by Eagle Biosciences

The Vedolizumab(Entyvio®)Antibodies Elisa Assay can be found in Eagle Biosciences’ Drug Monitoring Assay Kit Line. The Drug Monitoring Assay Kit line offers a wide range of kits for the to measure the presence and effectiveness of a wide rang of drugs in the body.

Our Vedolizumab Antibodies Elisa Assay measures the presence of anti-Verdolizumab antibodies in the blood.

What are the benfits to measuring Vedolizumab Antibodies?

The drug Vedolizumab is a humanised immunoglobulin G1 monoclonal antibody that binds exclusively to the lymphocyte integrin α4β7. It is used for the treatment of moderately to severely ulcerative colitis [UC] or Crohn’s disease [CD]. During treatment, the presence of persistent anti-Vedolizumab antibody can be observed to substantially reduce serum concentrations of Vedolizumab in the bloodstream, either to undetectable or negligible levels, reducing the effectiveness of the treatment.

The Eagle Biosciences Antibody to Vedolizumab ELISA Assay kit has been designed for the measurement of free antibodies against this drug to detect treatment effectiveness.

 

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Calretinin ELISA: A New Assay for the Detection of Mesothelioma

by Eagle Biosciences

Mesothelioma

Two million tons of asbestos are still being manufactured annually. Every year over 100,000 people die from cancer diseases caused by being exposed to asbestos. One common type of asbestos-related cancer causing disease is mesothelioma.

The gold standard for diagnosing mesothelioma is biopsies. These procedures can often be invasive and due to the long latency period of mesothelioma (on average it takes 20-40 years before symptoms of the disease to occur for diagnosis) a diagnosis is often made in the later stages of the disease, but early detection is key for the treatment of mesothelioma. 

Calretinin is a calcium binding protein expressed in neurons of the central and peripheral nervous systems of the body, as well normal body tissues such as the mesothelium cells that are affected by asbestos causing mesothelioma. Research has shown that calretinin is a useful biomarker for distinguishing malignant mesothelial tumors.

The new Calretinin ELISA Assay was developed by the cooperation of DLD Diagnostika and the Institute for Prevention and Occupational Medicine of the German Social Accident Insurance of the Ruhr-University Bochum (IPA).

This assay is highly specific, sensitive and applicable for the early detection of human calretinin. The new test is validated in large case-control studies and is also involved in a prospective study. 

 

Carletinin will add to a list of possible candidates for a biomarker panel; the more markers present the more marker combination with possible advantages there are to choose from. 

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All About Endostatin

by Eagle Biosciences

What is ENDOSTATIN?


Endostatin is the biologically active 20-kDa
C-terminal fragment of collagen XVIII.

Which function does it have?Endostatin is a highly potent inhibitor of
angiogenesis: it induces microvascular endothelial cell
apoptosis and inhibits endothelial proliferation and angiogenesis.

What is the clinical relevance
of Endostatin? Remodelling of the extracellular matrix is
present in various types of renal disease e.g. in renal carcinoma and fibrosis.
Endostatin is currently being tested as an anti-angiogenic drug.

Endostatin levels
are associated with (click here to view summary of studies):



– kidney injury in the elderly
– cardiovascular events and survival in CKD subjects
– kidney disease and mortality in patients with type 2 Diabetes
– Endostatin may improve acute kidney injury risk prediction in critically
ill subjects


How can I measure
Endostatin?

Endostatin
can easily be measured with our highly specific Endostatin ELISAs

Human ENDOSTATIN ELISA

for serum, plasma & urine

sample volume: 10 µl / well

Assay Specifications

Assay Validation Data

Instructions for Use

Mouse/Rat ENDOSTATIN ELISA


for serum and plasma
sample volume: 5 µl / well

Assay Specifications

Assay Validation Data

Instruction for Use

Features and Benefits:

SUPERIOR QUALITY – kits fully
validated (internat. quality guidelines)

HIGHLY SPECIFIC KITS
– utilizing characterized antibodies

EASY
handling – all
reagents included in the kits

LOW sample volume

FAST – results in 3
hours

Get a
20% Discount on Endostatin Kits*

*Promotion is valid through June 30, 2017.
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EagleBio Participates in 3rd Annual Gate City Marathon and Relay

by Eagle Biosciences


                 EagleBio Senior Team 

EagleBio participated in the 3rd annual Gate City Marathon & Relay on Sunday, May 21, 2017.  This is EagleBio’s 3rd year participating with a relay team full of rock stars!! Our group ran in the race as a 5 leg relay on a 26.2-mile course that took runners past historic former mills, rivers, parks and neighborhoods. 

This team once again amazed their fans and trimmed off 27 minutes from last year’s time! What a race day it was with fantastic weather, great course, helpful officials, and supportive crowd!  



                        EagleBio Junior Team: NYOKO

We were also very lucky to have a 2nd relay team again this year, the Junior EagleBio Team: NYOKO! This team was outstanding, they won FIRST PLACE out of all the relay teams and yes they beat the senior team above (by a lot)!

Great job, NYOKO!

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Nebraska Study Utilized EagleBio’s Secretory IgA (sIgA) ELISA

by Eagle Biosciences

Image 1

 

EagleBio’s Secretory
IgA (SIgA) ELISA Assay Kit was recently used in a Nebraska study. This assay is part of our Immunology Assay Kit line which is comprised of a unique,versatile set of products for a variety
of applications, research, and fields of study.
Check the product pages for full details on the kit or view the links
below to find a new publication referencing our Secretory
IgA (SIgA) ELISA Assay Kit.

Morrow, J. et.
al. Assessing the Archaeoparasitological
Potential of Quids As a Source Material for Immunodiagnostic Analyses. Korean
J Parasitol. 2016 Oct; 54(5): 605–616. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127539/

“An additional
ELISA test targeting secretory IgA (sIgA) was conducted to further examine the
failure to detect parasite-induced antibodies from quids. Herein, the methods
used for quid preparation and ELISA procedures are described so that they can
be further developed by future researchers. The results are discussed in light
of the potential future of quid analysis.” Read More

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New Product Introduction: iLite FGF-21 Assay Ready Cells

by Eagle Biosciences

iLite® FGF-21 Activity Assays

First products available NOW, many more coming soon!

Image result for Euro Diagnostica

iLite® FGF-21 Assay Ready Cells

The iLite® FGF21 Assay Ready Cells can be used in an assay for the quantification of the potency of analogues of human FGF21, the potency of FGFR1c antagonists or a neutralizing antibody response against such products in human serum in the absence of serum matrix effects.

Developed by:

Key benefits of iLite® FGF-21 Assay Ready Cells:

  • Unparalleled sensitivity (>10 fold inductions)
  • Highly specific reporter gene cell lines
  • Normalization read-out included
  • Negative control available for screening of unspecific activity
  • Easy to use – ready-to-use from the freezer, without culturing of cells

LIMITED OFFER!

50% off on your first iLite® FGF-21 Assay Ready Cells
Use Code: ADCC50 

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Study Shows How BPA May Affect Inflammatory Breast Cancer

by Eagle Biosciences

        image credit: https://corporate.dukehealth.org

DURHAM, N.C. — The chemical bisphenol A, or BPA, appears to aid the survival of inflammatory breast cancer cells, revealing a potential mechanism for how the disease grows, according to a study led by researchers in the Department of Surgery at Duke University School of Medicine and the Duke Cancer Institute.

Read More


Source: (https://corporate.dukehealth.org)

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Webinar: Unique, Early Biomarker for Cell Proliferation

by Eagle Biosciences

 

Eagle Biosciences and AroCell are pleased to invite you to a
webinar on the application of the TK
210 (Thymidine Kinase 1) ELISA in the study of malignancies.

There are many biomarkers of tumor mass, but few serum
biomarkers of tumor proliferation, a key indicator when making prognoses
and choosing and monitoring therapy.

The assay of serum Thymidine Kinase 1 with the AroCell TK 210
ELISA has the potential to be such a biomarker. There is no cost for
this event.

 

When: March
23, 2017 | 11 am to 11:30 am EDT

Presenters:

  • Staffan Eriksson, Professor in Medical and Physiological Chemistry, Swedish
    University of Agricultural Sciences
  • Martin
    Shaw    , Business Development Manager at AroCell.

Key points of the presentation will be:

  • Thymidine
    kinase 1 (TK1) is a well-known proliferation biomarker but most
    current methods are based on enzyme activity methods and may
    underestimate serum TK1 levels, particularly in subjects with solid
    tumors
  • The AroCell
    TK 210 ELISA adds value to studies with existing tumor biomarkers
  • Data will be
    presented on the value of the AroCell TK 210 ELISA in the study of a
    range of hematological and solid tumors.

Receive a white paper on the use of TK1 and the AroCell TK210 ELISA in studying solid tumors HERE

 

 

About AroCell

AroCell AB was founded in 2003. The company is located in Uppsala, Sweden and develops and commercializes tests that can be used in the prognosis, monitoring and follow-up of cancer.