Zonulin Stool ELISA Assay Kit
Zonulin Stool ELISA Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 0.241 ng/mL
Dynamic Range: 0.25 – 64 ng/mL
Incubation Time: 2.5 hours
Sample Type: Stool
Sample Size: 15 mg
Alternative Names: Zonulin ELISA (Stool), Human Zonulin Stool ELISA
For Research Use Only
Based on Immundiagnostik studies of stool samples of apparently healthy persons (n = 40), a median value of 61ng/ml (± 46ng/ml) was estimated. We recommend each laboratory to establish its own reference range.
Assay Background for Zonulin Stool ELISA Assay Kit
Zonulin is a novel human protein analogue to the zonula occludens toxin derived from Vibrio cholerae which participates in tight junctions between cells of the wall of the digestive tract. Zonulin binds to a specific receptor on the surface of intestinal epithelia and triggers a cascade of biochemical events which induces tight junction disassembly and a subsequent permeability increase of the intestinal epithelia, allowing some substances to pass through and activate immune reactions.
Fasano and his co-workers found that the zonulin-zonulin-receptor-system is more activated in celiac disease and type 1 diabetes mellitus patients. Patients with active celiac disease showed higher levels of zonulin and anti-zonulin antibodies compared to non-celiac patients and patients in remission, who were on a gluten-free diet.
Concerning the autoimmune type 1 diabetes, in experiments with rats it could be demonstrated that elevated zonulin levels as well as increased intestinal permeability precede a type 1 diabetes disease. Conversely, type 1 diabetes could be prevented by inhibition of zonulin in animal experiments.
In addition, it was reported that many people who suffer from celiac disease also suffer from other autoimmune disorders. It is suggested that increased levels of zonulin are a contributing factor to the development of celiac disease and other autoimmune disorders such as insulin dependent diabetes, multiple sclerosis and rheumatoid arthritis.