Vitamin B6 Microtiter Plate Assay Kit


The Eagle Biosciences Vitamin B6 Assay is a microtiter plate test kit based on a microbiological assay which measures the vitamin B6 content (pyridoxine, pyridoxal, pyridoxamine) in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the vitamin B6 content. The Vitamin B6 Microtiter Plate Assay is for research use only. 

SKU: KIFR006 Categories: , ,

Vitamin B6 Microtiter Plate Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.36 µg/L
Dynamic Range: 0.36 – 3 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 300 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

The vitamin B6 group comprises three natural forms: pyridoxine, pyridoxamine and pyridoxal, which, during metabolism, are converted to the enzymatically active form pyridoxal-5-phosphate (P5P).Pyridoxal-5-phosphate (here described as “vitamin B6”) is a cofactor in more than a hundred enzyme reactions. One of its functions is to perform transamination, a key step in both breaking down and producing amino acids in the body. Vitamin B6 is also necessary for the synthesis of neurotransmitters, as well as hemoglobin in red blood cells. And vitamin B6 plays a central role in fat metabolism.

Assay Principle

The serum samples are enzymatically pre-treated in order to determine the total vitamin B6 content. The diluted samples are transferred in the wells of a microtiter plate [PLATE] coated with Saccharomyces cerevisiae which metabolizes vitamin B6. The addition of vitamin B6 in either standards [STD], controls [CTRL] or samples gives a vitamin B6-dependent growth response until vitamin B6 is consumed. After incubation at 30°C for 44 – 48 hours, the growth of Saccharomyces cerevisiae is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B6 is directly proportional to the turbidity.


Product Manual