Vitamin B6 Microtiter Plate Assay

$920.00

The Vitamin B6 Assay is a microtiter plate test kit based on a microbiological assay which measures the vitamin B6 content (pyridoxine, pyridoxal, pyridoxamine) in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the vitamin B6 content. The Vitamin B6 Microtiter Plate Assay is for research use only.

SKU: KIFR006 Categories: , ,

Vitamin B6 Microtiter Plate Assay

Vitamin B6 Microtiter Plate Assay manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Sensitivity: 0.36 µg/L
Dynamic Range: 0.36 – 3 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 300 µL
Alternative Names: Pyridoxine, pyridoxamine and pyridoxal
For Research Use Only
Controls Included


Preparation and Storage
– Store test kit / reagents at 2-8° C.
– Prepare reagents freshly and use immediately after preparation. Discard remaining unused reagents and waste in accordance with country,federal,state,and local regulations.
– Put unused reagents(standard,medium)in the test kit and store at 2-8° C.
– Store unused strips in the original package with dry bag securely closed at 2-8° C to prevent contamination or moisture exposure.
– No warranty can be given after the expiry date (see label of test package).
– To run assay more than once,ensure that reagents are stored at conditions stated on the label.Prepare only the appropriate amount necessary for each assay.The kit can be used up to 4 times within the expiry date stated on the label.


Assay Background

The vitamin B6 group comprises three natural forms: pyridoxine, pyridoxamine and pyridoxal, which, during metabolism, are converted to the enzymatically active form pyridoxal-5-phosphate (P5P).Pyridoxal-5-phosphate (here described as “vitamin B6”) is a cofactor in more than a hundred enzyme reactions. One of its functions is to perform transamination, a key step in both breaking down and producing amino acids in the body. Vitamin B6 is also necessary for the synthesis of neurotransmitters, as well as hemoglobin in red blood cells. And vitamin B6 plays a central role in fat metabolism.
Vitamin B6 deficiency symptoms:
– Disturbance of protein biosynthesis
– Muscular weakness,loss of muscle control
– Skin disorders(dermatitis,pigment abnormality)
– Nervous disorders(irritability,depression,palsy)
– Insomnia
Vitamin B6 deficiency can be considered as a risk factor for myocardial infarction, peripheral vascular diseases and atherosclerosis,especially in connection with the regulation of homocysteine metabolism.

Vitamin B6 deficiency can result from:
– Chronic inflammatory bowel diseases(colitis ulcerosa, Crohn’s disease, gluten sensitivity)
– Dialysis
– Alcohol abuse
– Homocysteinuria
– Pregnancy and lactation


Products Related to Vitamin B6 Microtiter Plate Assay

Vitamin B1 Microtiter Plate Kit
Vitamin B12 Microtiter Plate Assay
Vitamin B2 Serum Microtiter Plate Assay Kit

Additional Information

Assay Principle


The serum samples are enzymatically pre-treated in order to determine the total vitamin B6 content. The diluted samples are transferred in the wells of a microtiter plate [PLATE] coated with Saccharomyces cerevisiae which metabolizes vitamin B6. The addition of vitamin B6 in either standards [STD], controls [CTRL] or samples gives a vitamin B6-dependent growth response until vitamin B6 is consumed. After incubation at 30°C for 44 – 48 hours, the growth of Saccharomyces cerevisiae is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B6 is directly proportional to the turbidity.

Manual

Product Manual