Vitamin B6 Microtiter Plate Assay
Vitamin B6 Microtiter Plate Assay manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 0.36 µg/L
Dynamic Range: 0.36 – 3 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 300 µL
Alternative Names: Pyridoxine, pyridoxamine and pyridoxal
For Research Use Only
Preparation and Storage
– Store test kit / reagents at 2-8° C.
– Prepare reagents freshly and use immediately after preparation. Discard remaining unused reagents and waste in accordance with country,federal,state,and local regulations.
– Put unused reagents(standard,medium)in the test kit and store at 2-8° C.
– Store unused strips in the original package with dry bag securely closed at 2-8° C to prevent contamination or moisture exposure.
– No warranty can be given after the expiry date (see label of test package).
– To run assay more than once,ensure that reagents are stored at conditions stated on the label.Prepare only the appropriate amount necessary for each assay.The kit can be used up to 4 times within the expiry date stated on the label.
The serum samples are enzymatically pre-treated in order to determine the total vitamin B6 content. The diluted samples are transferred in the wells of a microtiter plate [PLATE] coated with Saccharomyces cerevisiae which metabolizes vitamin B6. The addition of vitamin B6 in either standards [STD], controls [CTRL] or samples gives a vitamin B6-dependent growth response until vitamin B6 is consumed. After incubation at 30°C for 44 – 48 hours, the growth of Saccharomyces cerevisiae is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B6 is directly proportional to the turbidity.
Vitamin B6 deficiency can result from:
– Chronic inflammatory bowel diseases(colitis ulcerosa, Crohn’s disease, gluten sensitivity)
– Alcohol abuse
– Pregnancy and lactation