Vitamin B6 Microtiter Plate Assay
Vitamin B6 Microtiter Plate Assay manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 0.36 µg/L
Dynamic Range: 0.36 – 3 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 300 µL
Alternative Names: Pyridoxine, pyridoxamine and pyridoxal
For Research Use Only
Controls Included
Preparation and Storage
– Store test kit / reagents at 2-8° C.
– Prepare reagents freshly and use immediately after preparation. Discard remaining unused reagents and waste in accordance with country,federal,state,and local regulations.
– Put unused reagents(standard,medium)in the test kit and store at 2-8° C.
– Store unused strips in the original package with dry bag securely closed at 2-8° C to prevent contamination or moisture exposure.
– No warranty can be given after the expiry date (see label of test package).
– To run assay more than once,ensure that reagents are stored at conditions stated on the label.Prepare only the appropriate amount necessary for each assay.The kit can be used up to 4 times within the expiry date stated on the label.
Assay Background
The vitamin B6 group comprises three natural forms: pyridoxine, pyridoxamine and pyridoxal, which, during metabolism, are converted to the enzymatically active form pyridoxal-5-phosphate (P5P).Pyridoxal-5-phosphate (here described as “vitamin B6”) is a cofactor in more than a hundred enzyme reactions. One of its functions is to perform transamination, a key step in both breaking down and producing amino acids in the body. Vitamin B6 is also necessary for the synthesis of neurotransmitters, as well as hemoglobin in red blood cells. And vitamin B6 plays a central role in fat metabolism.
Vitamin B6 deficiency symptoms:
– Disturbance of protein biosynthesis
– Muscular weakness,loss of muscle control
– Skin disorders(dermatitis,pigment abnormality)
– Nervous disorders(irritability,depression,palsy)
– Insomnia
Vitamin B6 deficiency can be considered as a risk factor for myocardial infarction, peripheral vascular diseases and atherosclerosis,especially in connection with the regulation of homocysteine metabolism.
Vitamin B6 deficiency can result from:
– Chronic inflammatory bowel diseases(colitis ulcerosa, Crohn’s disease, gluten sensitivity)
– Dialysis
– Alcohol abuse
– Homocysteinuria
– Pregnancy and lactation
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