Pancreatic Amylase ELISA Assay Kit

$615.00

This Pancreatic Amylase ELISA Assay Kit is an enzyme immunoassay intended for the quantitative determination of pancreatic amylase in stool. The Eagle Biosciences Pancreatic Amylase ELISA Assay Kit is for research use only and should not be used for diagnostic procedures.

SKU: KR6410 Categories: ,

Pancreatic Amylase ELISA Assay Kit

Pancreatic Amylase ELISA Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Dynamic Range: 440-28000 mU/l
Sensitivity: 237 mU/I
Incubation Time: 1 hour, 10-20 minutes
Sample Type: Stool
Sample Size: 15 mg
Alternative Names: Human Pancreatic Amylase ELISA, PAMY ELISA
For Research Use Only

Controls Included


Quality Control and Reference Ranges
It is recommended to use external controls for internal quality control, if possible. Control samples should be analysed with each run. Results, generated from the analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the patient samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits.
Reference range:
Pancreatic amylase: > 1000U/l
Grey area 1000–1500U/l
We recommend each laboratory to establish its own reference range.


Assay Principle

This Pancreatic Amylase ELISA Assay Kit is designed for the determination of pancreatic amylase in stool. In a first incubation step, the pancreatic amylase from the sample is bound to the specific anti-pancreatic amylase antibodies coated on the plate. To remove all unbound sub- stances, a washing step is carried out. In a further incubation step, peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of pancreatic amylase in the sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. Pancreatic amylase, present in the patient samples, is determined directly from this curve.


Related Products

Pancreatic Elastase ELISA
PMN-Elastase (Serum/Plasma) ELISA Assay
PMN-Elastase (Stool) ELISA Kit

Additional Information

Assay Background


Like lipase and elastase, the pancreatic amylase is synthesised in the pancreas. In case of a chronic pancreatitis, pancreatic amylase is decreased. Some forms of disease (alcoholism, accidental trauma) are associated with a pancreatic insufficiency. In the routine laboratory, pancreatic amylase has proved itself to be a genuine alternative to elastase-I in stool diagnosis.

Manual

Product Manual