Vitamin B2 Microtiter Plate Assay Kit (Whole Blood)


The Eagle Biosciences Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.

SKU: KIFR002VB Categories: , ,

Vitamin B2 Microtiter Plate Assay Kit (Whole Blood)

For Research Use Only

Size: 1×96 wells
Sensitivity: 5 µg/L
Dynamic Range: 5 – 60 µg/L
Incubation Time: 48 hours
Sample Type: Whole Blood
Sample Size: 100 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

Vitamin B2 is a component of the flavin-nucleotides and as such is involved in the transport of hydrogen ions and electrons. It is an essential coenzyme for the metabolism of carbohydrates, fats, and proteins. The biological form of vitamin B2 is riboflavin-5-phosphate. The most important vitamin B2 derivatives are flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD). In blood, about 60% of FAD and FMN are protein bound; only about 0.5 – 2% occur in free form.Riboflavin-5-phosphate, FMN, and FAD are transported in the plasma by a variety of proteins, including albumin, fibrinogen,riboflavin-binding protein and other globulins. Although vitamin B2 is eliminated regularly in the urine, its determination in urine samples is not recommended because of fluctuations in the concentration.

Assay Principle

Pretreated EDTA whole blood samples are transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusrhamnosus. The addition of Vitamin B2 in either standards [STD] or samples give a Vitamin B2-dependent growth response until Vitamin B2 is consumed. After incubation at 37°C for 48 hours, the growth of Lactobacillusrhamnosus is measured turbidimetrically at 610 – 630nm (alternatively at 540 – 550nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of Vitamin B2 is directly proportional to the turbidity.


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