Human TRAIL R4 ELISA Assay


The Human TRAIL R4 ELISA Assay is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of TRAIL R4/DcR2 in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human TRAIL R4/DcR2. The Eagle Biosciences Human TRAIL R4/DCR2 ELISA Assay kit is for Research Use Only.

SKU: C6431-K01 Categories: , ,

Human TRAIL R4 ELISA Assay

The Human TRAIL R4 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 64 pg/ml
Dynamic Range: 312.5 pg/ml – 10000 pg/ml
Incubation Time: 3h 40 min
Sample Type: Serum, Cell lysates
Sample Size: 100 µl
Alternative Names: CD264, TRUNND, DcR2

Assay Background

Human Trail R4, also called DcR2, CD264 or TRUNND is a type 1 TNF R family membrane protein which is a receptor for TRAIL. In the Trail receptor family, Trail R4 (DcR2) but also Trail R3 (DcR1) antagonize TRAIL-induced apoptosis whereas Trail R1 (DR4) and Trail R2 (DR5) transducer an apoptosis signal. Trail R4 has a truncated intracellular domain.  Overexpression of Trail R4 has been shown to protect cells bearing Trail R1 and/or Trail R2 from Trail-induced apoptosis. Many tumor cell lines don’t express the decoy receptors and are therefore sensitive to TRAIL if they express Trail R1 and/or Trail R2.

Storage Instructions
Store the Human TRAIL R4/DCR2 ELISA kit reagents between 2 and 8°C. Immediately after use remaining reagents should be returned to cold storage (2-8°C). Expiry of the kit and reagents is stated on box front labels. The expiry of the Human TRAIL R4/DCR2 ELISA Assay kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.

Related Products

Human TRAIL R1 ELISA Assay
Human TRAIL R2 ELISA Assay
Human TRAIL R3 ELISA Assay

Additional Information

Assay Principle

A capture Antibody highly specific for the Eagle Biosciences Human TRAIL R4 ELISA Assay has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of TRAIL R4 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti- TRAIL R4 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of TRAIL R4 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of TRAIL R4 in any sample tested.

Typical Standard Curve

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