Vitamin B2 Serum Microtiter Plate Assay Kit
Vitamin B2 Serum Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 5 µg/L
Dynamic Range: 5 – 60 µg/L
Incubation Time: 24 hours
Sample Type: Serum
Sample Size: 150 µL
For Research Use Only
Controls Included
Preparation and Storage:
- Store test kit / reagents at 2-8°C.
- Prepare reagents freshly and use immediately after preparation. Discard remaining unused reagents and waste in accordance with country, federal, state, and local regulations.
- Put unused reagents(standard,medium)in the test kit and store at 2-8°C.
- Store unused strips in the original package with dry bag securely closed at 2-8° C to prevent contamination or moisture exposure.
- No warranty can be given after the expiry date (see label of test package).
- To run assay more than once, ensure that reagents are stored at conditions stated on the label. Prepare only the appropriate amount necessary for each assay.The kit can be used up to 4 times within the expiry date stated on the label.
Assay Background
Vitamin B2 (riboflavin), as flavin mononucleotide (FMN) or flavin adenine dinucleotide (FAD), acts as an essential coenzyme in many oxidation-reduction reactions. Flavins are critical for the metabolism of carbohydrates, fats, and proteins.FAD is part of the electron transport (respiratory) chain, which is central to energy production. In conjunction with cytochrome P-450, flavins also participate in the metabolism of drugs and toxins.
Riboflavin is unique among the water-soluble vitamins in that milk and dairy products make the greatest contribution to its intake in Western diets. Biochemical signs of depletion arise within only a few days of dietary deprivation. Poor riboflavin status in Western countries seems to be of most concern for the elderly and adolescents, despite the diversity of riboflavin-rich foods available. Deficiency results in oral, ocular, cutaneous, and genital lesions. Primary riboflavin deficiency is associated with inadequate consumption of milk and other animal products. Secondary deficiencies are most common in chronic diarrheas, liver disease, chronic alcoholism, and postoperative situations in which nutrient infusions lack supplementary vitamins.
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