Vitamin ELISA Assay Kit

Vitamin B2 Serum Microtiter Plate Assay Kit

$1,065.00

The Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. The Vitamin B2 Assay kit is For Research Use only.

SKU: KIFR002S Categories: , ,

Vitamin B2 Serum Microtiter Plate Assay Kit

Vitamin B2 Serum Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Sensitivity: 5 µg/L
Dynamic Range: 5 – 60 µg/L
Incubation Time: 24 hours
Sample Type: Serum
Sample Size: 150 µL
For Research Use Only
Controls Included

Preparation and Storage:

  • Store test kit / reagents at 2-8°C.
  • Prepare reagents freshly and use immediately after preparation. Discard remaining unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Put unused reagents(standard,medium)in the test kit and store at 2-8°C.
  • Store unused strips in the original package with dry bag securely closed at 2-8° C to prevent contamination or moisture exposure.
  • No warranty can be given after the expiry date (see label of test package).
  • To run assay more than once, ensure that reagents are stored at conditions stated on the label. Prepare only the appropriate amount necessary for each assay.The kit can be used up to 4 times within the expiry date stated on the label.

Assay Background

Vitamin B2 (riboflavin), as flavin mononucleotide (FMN) or flavin adenine dinucleotide (FAD), acts as an essential coenzyme in many oxidation-reduction reactions. Flavins are critical for the metabolism of carbohydrates, fats, and proteins.FAD is part of the electron transport (respiratory) chain, which is central to energy production. In conjunction with cytochrome P-450, flavins also participate in the metabolism of drugs and toxins.

Riboflavin is unique among the water-soluble vitamins in that milk and dairy products make the greatest contribution to its intake in Western diets. Biochemical signs of depletion arise within only a few days of dietary deprivation. Poor riboflavin status in Western countries seems to be of most concern for the elderly and adolescents, despite the diversity of riboflavin-rich foods available. Deficiency results in oral, ocular, cutaneous, and genital lesions. Primary riboflavin deficiency is associated with inadequate consumption of milk and other animal products. Secondary deficiencies are most common in chronic diarrheas, liver disease, chronic alcoholism, and postoperative situations in which nutrient infusions lack supplementary vitamins.

Products Related to Vitamin B2 Serum Assay

Vitamin B2 Microtiter Plate Assay (Whole Blood)
Vitamin B1 Microtiter Plate Kit
Vitamin B6 Microtiter Assay Kit

Additional Information

Assay Principle

Serum samples are transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusrhamnosus. The addition of Vitamin B2 in either standard [STD] or samples gives a Vitamin B2-dependent growth response until Vitamin B2 is consumed. After incubation at 37°C for 48 hours, the growth of Lactobacillusrhamnosus is measured turbidimetrically at 610 – 630nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of Vitamin B2 is directly proportional to the turbidity.

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