Niacin Microtiter Plate Assay Kit


The Niacin Microtiter Plate Assay is a microtiter plate test kit based on a microbiological assay which measures the total niacin content (nicotinic acid and nicotinamide) in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the niacin content. The Niacin Assay kit is For Research Use Only.

SKU: KIFR003 Categories: , ,

Niacin Microtiter Plate Assay Kit

Niacin Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Sensitivity: 2 µg/L
Dynamic Range: 2 – 40 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 100 µL
For Research Use Only
Controls Included

Assay Principle

In the Niacin Microtiter Plate Assay Kit, serum samples are diluted and added into the microtiter plate wells coated with Lactobacillus plantarum which metabolizes niacin. The presence of niacin both in standards [STD] and samples gives a niacin-dependent growth response. After incubation at 37°C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 – 630 nm (alternative at 540 – 550 nm) in an ELISA-reader. A dose-response curve of absorbance unit (optical density, OD at 610 nm) vs. concentration is generated using the values obtained from the standard. Niacin present in the patient samples is determined directly from this curve.

Niacin deficiency syndromes:
Symptoms of minor niacin deficiency:
– Loss of appetite
– Depression
– Dementia
– Insomnia
– Weakness
– Irritability

Severe niacin deficiency may cause pellagra. The term pellagra is derived from the Italian words“pelle agra” meaning “rough” or “smarting skin”. Pellagra is characterized by symptoms such as:
– Glossitis
– Sore, swollen, purple-red tongue
– Skin lesions primarily located on sun-exposed areas
Niacin as a cholesterol lowering drug:
Niacin increases HDL cholesterol and reduces LDL cholesterol and triglycerides. When taken in conjunction with another cholesterol medication, diet or exercise, niacin has been proven to reduce “bad” cholesterol levels. A niacin-stat in combination therapy substantially improves 4 major lipoprotein levels associated with atherosclerotic disease (Insull et al. 2004). The drug combination had good records in clinical trials for reduction in cardiovascular events and improvement in progression/regression of coronary lesions.
– Deeply pigmented skin on sun-exposed areas
– Alcohol abuse
– Dementia
– Dry skin and mouth
– Numbness of the extremities
– Inflammation of the mucous membranes of the tongue and mouth
– Digestive disorders
Niacin can be synthesized in the body from tryptophan,whereby the conversion requires the presence of thiamine, pyridoxine, and riboflavin. Any deficiency in these vitamins can affect the niacin metabolism.

Related Products

Pantothenic Acid Microtiter Plate Assay
Folic Acid Microtiter Plate Kit
Biotin Microtiter Plate Assay

Additional Information

Assay Background

Niacin (nicotinic acid and nicotinamide) is used by the body to form coenzymes such as nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+). As many as 200 enzymes require the two coenzymes, NAD+ and NADP+, mainly to accept or donate electrons for redox reactions. NAD+ functions most often in reactions involving the degradation (catabolism) of carbohydrates, fats, proteins, and alcohol to produce energy. NADP+ functions more often in biosynthetic (anabolic) reactions, such as in the synthesis of fatty acids and cholesterol. Since almost every metabolic pathway uses either NAD+ or NADP+, it is not surprising to find signs and symptoms of niacin deficiency in severe metabolic disorders. The worst of these is pellagra which is characterized by the four D’s, representing: Dermatitis, Diarrhea, Dementia, and Death.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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