Niacin Microtiter Plate Assay Kit


The Eagle Biosciences Niacin is a microtiter plate test kit based on a microbiological assay which measures the total niacin content (nicotinic acid and nicotinamide) in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the niacin content. The Niacin Assay kit is For Research Use Only.

SKU: KIFR003 Categories: , ,

Niacin Microtiter Plate Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 2 µg/L
Dynamic Range: 2 – 40 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 100 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

Niacin (nicotinic acid and nicotinamide) is used by the body to form coenzymes such as nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+). As many as 200 enzymes require the two coenzymes, NAD+ and NADP+, mainly to accept or donate electrons for redox reactions. NAD+ functions most often in reactions involving the degradation (catabolism) of carbohydrates, fats, proteins, and alcohol to produce energy. NADP+ functions more often in biosynthetic (anabolic) reactions, such as in the synthesis of fatty acids and cholesterol. Since almost every metabolic pathway uses either NAD+ or NADP+, it is not surprising to find signs and symptoms of niacin deficiency in severe metabolic disorders. The worst of these is pellagra which is characterized by the four D’s, representing: Dermatitis, Diarrhea, Dementia, and Death.

Assay Principle

Serum samples are diluted and added into the microtiter plate wells coated with Lactobacillusplantarum which metabolizes niacin. The presence of niacin both in standards [STD] and samples gives a niacin-dependent growth response. After incubation at 37°C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 – 630 nm (alternative at 540 – 550nm) in an ELISA-reader. A dose-response curve of absorbance unit (optical density, ODat 610nm) vs. concentration is generated using the values obtained from the standard. Niacin present in the patient samples is determined directly from this curve.


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