Vitamin B1 Microtiter Plate Assay Kit
Vitamin B1 Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 3 µg/L
Dynamic Range: 3 – 15 µg/L
Incubation Time: 24 hours
Sample Type: Whole Blood
Sample Size: 100 µL
Alternative Names: Thiamin, thiamine pyrophosphate, thiamine
For Research Use Only
Vitamin B1 concentration was determined in 42 samples of blood donors. The median value of this set of numbers was 48.1 (44.3) µg/ L. The array of 2-SD was 30- 66 µg/L.
Please note: A concentration range of 30-150 µg/l Vitamin B1 is covered at a sample dilution 1 :10. We recommend each laboratory to develop its own normal range as normal ranges depend on the choice of patient collective. The values mentioned above are only for orientation and can deviate from other published data.
The blood samples are enzymatically pre-treated and then transferred in the wells of a microtiter plate[PLATE] coated with Lactobacillusfermentum. The addition of vitamin B1 in either standard [STD], control [CTRL1, CTRL2] or samples gives a vitamin B1-dependent growth response until vitamin B1 is consumed. After incubation at 37 °C for 48 hours, the growth of Lactobacillus fermentum is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B1 is directly proportional to the turbidity.