Urinary Cortisol ELISA Assay


The Urinary Cortisol ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of free Cortisol concentration in urine. The Urinary Cortisol ELISA Assay Kit is for research use only and is not intended for diagnostic procedures.

SKU: DCM018 Categories: , ,

Urinary Cortisol ELISA Assay Kit

The Urinary Cortisol ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.22 ng/mL
Dynamic Range: 1 – 200 ng/ml
Incubation Time: 1.5 hours
Sample Type: Urine
Sample Size: 10 µL

Controls Included

Reference Values
Normal Range (24 hours): 1.5 – 63µg

Assay Background

Cortisol is a steroid hormone released from the adrenal cortex in response to an hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system.

Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid-binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors.

These normal endogenous functions are the basis for the physiological consequences of chronic stress – prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.

The free cortisol fraction represents the metabolically active cortisol. In normal conditions, less then 1% it comes excrete in urines. In pathological conditions (syndrome of Cushing) the levels of free urinary cortisolo are elevate, because the CBG don’t bound the plasmatic cortisol in excess and it was remove with urines.

During pregnancy or estro-progestogen treatment an increase of plasmatic cortisol caused by an increment of the production of the transport protein, but the levels of free urinary cortisol results normal to indicate a correct surrenic functionality. This test is very useful to estimate the real surrenic function, because is dose the free cortisol, it is the metabolically active form. Moreover the measurement of free urinary cortisol is the better parameter for the diagnosis of the Cushing’s syndrome.

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Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Principle

The Cortisol (antigen) in the sample competes with the antigenic Cortisol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Cortisol coated on the microplate (solid phase).

After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added.

The color intensity is inversely proportional to the Cortisol concentration of in the sample.Cortisol concentration in the sample is calculated through a calibration curve.

Comparison of ELISA vs. LC-MS/MS

DCM018 Urinary Cortisol ELISA LC/MS Comparison

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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