Urinary Cortisol ELISA Assay Kit
The Urinary Cortisol ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.22 ng/mL
Dynamic Range: 1 – 200 ng/ml
Incubation Time: 1.5 hours
Sample Type: Urine
Sample Size: 10 µL
Normal Range (24 hours): 1.5 – 63µg
Cortisol is a steroid hormone released from the adrenal cortex in response to an hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress; it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system.
Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid-binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors.
These normal endogenous functions are the basis for the physiological consequences of chronic stress – prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.
The free cortisol fraction represents the metabolically active cortisol. In normal conditions, less then 1% it comes excrete in urines. In pathological conditions (syndrome of Cushing) the levels of free urinary cortisolo are elevate, because the CBG don’t bound the plasmatic cortisol in excess and it was remove with urines.
During pregnancy or estro-progestogen treatment an increase of plasmatic cortisol caused by an increment of the production of the transport protein, but the levels of free urinary cortisol results normal to indicate a correct surrenic functionality. This test is very useful to estimate the real surrenic function, because is dose the free cortisol, it is the metabolically active form. Moreover the measurement of free urinary cortisol is the better parameter for the diagnosis of the Cushing’s syndrome.