Cryptosporidium parvum Antigen ELISA Assay Kit
Cryptosporidium parvum Antigen ELISA Assay Kit Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2.5 hours
Sample Type: Stool
Sample Size: 50mg
For Research Use Only
The reproducibility of this Cryptosporidium Antigen ELISA is validated by measuring four samples (two negative and two positive) both in a single assay of 12-replicate determinations and in 6 different assays run on different dates. The results showed a consistent test results interpretation for all the samples.
This Fecal Cryptosporidium Antigen ELISA is a “sandwich” ELISA that is designed, developed and produced for the qualitative measurement of Cryptosporidium parvum antigen in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well.
Assay controls and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-Cryptosporidium parvum antibody on its wall. The Cryptosporidium parvum antigen will be bound to the antibody coated plate after an incubation period. The unbound matrices are washed away and a HRP-conjugated monoclonal antibody which specifically recognizes the protein of Cryptosporidium parvum is added for further immunoreactions. After an incubation period, an immunocomplex of “Anti-Cryptosporidium Antibody – Cryptosporidium parvum Antigen – HRP-conjugated Anti-Cryptosporidium Tracer Antibody” is formed if Cryptosporidium parvum antigen is present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to C. parvum proteins captured on the wall of each microtiter well is directly proportional to the amount of Cryptosporidium parvum antigen level in each test specimen.