Cryptosporidium parvum Antigen ELISA Assay Kit

$500.00

Eagle Biosciences Cryptosporidium parvum Antigen ELISA Assay Kit is intended for the qualitative detection of Cryptosporidium parvum antigen in feces. The Cryptosporidium parvum Antigen ELISA kit is for Research Use Only and is not intended for diagnostic or therapeutic purposes.

SKU: CRY35-K01 Categories: , ,

Cryptosporidium parvum Antigen ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2.5 hours
Sample Type: Stool
Sample Size: 50mg

Controls Included

Product Developed and Manufactured in the USA


Additional Information

Assay Background


Cryptosporidiosis is one of the main causes of persistent diarrhea in the developed world. It is caused by the presence of Cryptosporidium parvum oocysts in the gastro-intestinal tract.  This parasite is known to be highly pathogenic and its infectious stage is transmitted by faecal-oral contract. It is also an opportunistic pathogen found in immunocompromised patients.

The symptoms of cryptosporidiosis are watery diarrhea, stomach cramps, weight loss, nausea, and fever1. In industrialized countries, 2-2.5% of diarrhreal hospitalized patients shed C. parvum oocysts. Ten percent of AIDS patients have chronic cryptosporidiosis and this figure can be as high as 40% in certain developing countries.  C. parvum is diagnosed by either Ziehl-Neelsen stain or immunofluorescence in smears of unconcentrated specimens.

Assay Principle


This Eagle Biosciences Fecal Cryptosporidium Antigen ELISA is a  “sandwich” ELISA that is designed, developed and produced for the qualitative measurement of Cryptosporidium parvum antigen in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well. 

Assay controls and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-Cryptosporidium parvum antibody on its wall. The Cryptosporidium parvum antigen will be bound to the antibody coated plate after an incubation period. The unbound matrices are washed away and a HRP-conjugated monoclonal antibody which specifically recognizes the protein of Cryptosporidium parvum is added for further immunoreactions.  After an incubation period, an immunocomplex of “Anti-Cryptosporidium Antibody – Cryptosporidium parvum Antigen – HRP-conjugated Anti-Cryptosporidium Tracer Antibody” is formed if Cryptosporidium parvum antigen is present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to C. parvum proteins captured on the wall of each microtiter  well is directly proportional to the amount of Cryptosporidium parvum antigen level in each test specimen.

Specificity


The assay does not cross react to following organisms: Giardia, Rotavirus, and Adenovirus.

Manual

Product Manual