Pregnenolone ELISA Assay
The Pregnenolone ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.05 ng/mL
Dynamic Range: 0.1–25.6 ng/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 µL
Alternative Names: 3beta-hydroxypregn-5-en-20-one, Human Pregnenolone ELISA
The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of pregnenolone in the sample. A set of standards is used to plot a standard curve from which the concentration of pregnenolone in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE for Pregnenolone Assay
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.