Estradiol ELISA Assay Kit
The Estradiol ELISA Assay Kit is manufactured in the United States of America
Size: 1×96 wells
Sensitivity: 10 pg/mL
Dynamic Range: 20–3200 pg/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 µL
For Research Use Only
SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
The following compounds were tested for cross-reactivity with the Direct Estradiol ELISA kit with estradiol cross-reacting at 100%.
This assay should not be used for patients being treated with the drug fulvestrant (Faslodex®) which cross reacts with estradiol and could lead to a falsely elevated test result.
The principle of the Estradiol ELISA Assay Kit enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled estradiol (present in standards, controls and patient samples) and an enzyme-labelled estradiol (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of unlabeled estradiol in the sample. A set of standards is used to plot a standard curve from which the amount of estradiol in patient samples and controls can be directly read.