Estradiol ELISA Assay Kit


The Eagle Biosciences Estradiol ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of 17β-Estradiol concentration in human serum or plasma.  This Estradiol ELISA Assay ELISA Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM003 Categories: , ,

Estradiol ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 8.68 pg/mL
Dynamic Range: 20-2000 pg/ml
Incubation Time: 2.5 hour
Sample Type: Serum, Plasma
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Estradiol (17β-Estradiol) is a sex hormone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including bone structure. During the reproductive years most Estradiol in women is produced by the ovaries, smaller amounts of Estradiol are also produced by the adrenal cortex. In men, the testes produce Estradiol. In plasma Estradiol is largely bound to sex hormone binding globulin (SHBG), also to albumin, only a fraction is free and biologically active. Serum Estradiol measurement in women reflect primarily the activity of the ovaries. During pregnancy estrogen levels, including Estradiol, rise steadily towards term. Estradiol increases due to placental production. In adult premenopausal women, ovarian production of Estradiol is stimulated by luteinizing hormone (LH) and the follicle-stimulating hormone (FSH) during the menstrual cycle.

In adult women, Estradiol levels are measured in the evaluation of fertility and menstrual irregularities, and to monitor ovarian follicular function during induction of ovulation. In the female, Estradiol acts as a growth hormone for tissue of the reproductive organs. The development of secondary sexual characteristics in women is driven by Estradiol. Estradiol is involved also in man fertility. Estradiol regulates the bone maintenance. Post-menopause women experience an accelerated loss of bone mass due to a relative estrogen deficiency.

Estradiol affects the production of multiple proteins including lipoproteins, binding proteins, and proteins responsible for blood clotting. Estrogens have been found to have neuroprotective function. The Estradiol, for his activities, is involved in some types of cancer such as breast cancer and cancer of the uterine lining. In addition there are several benign gynecologic conditions that are dependent on estrogen such as endometriosis, leiomyomata uteri, and uterine bleeding.

Assay Principle

In the Estradiol ELISA Assay Kit, the 17β-Estradiol (antigen) in the sample competes with the antigenic 17β-Estradiol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti 17β-Estradiol coated on the microplate (solid phase).  After incubation, the bound/free separation is performed by a simple solid-phase washing. Then the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution (H2SO4) is added. The colour intensity is inversely proportional to the 17β-Estradiol  concentration  in the sample. 17β-Estradiol concentration in the sample is calculated through a calibration curve.


Product Manual



Bertucci, JI;Blanco, AM;Canosa, LF;Unniappan, S; Estradiol and testosterone modulate the tissue-specific expression of ghrelin, ghs-r, goat and nucb2 in goldfish, Gen. Comp. Endocrinol., 228:17-23; DOI 10.1016/j.ygcen.2016.01.006

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