Estradiol ELISA Assay Kit


The Estradiol ELISA Assay Kit is intended for the quantitative determination of Estradiol in human serum. The Estradiol ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: ESD31-K01 Categories: , ,

Estradiol ELISA Assay Kit

The Estradiol ELISA Assay Kit is manufactured in the United States of America

Size: 1×96 wells
Sensitivity: 10 pg/mL
Dynamic Range: 20–3200 pg/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 µL
For Research Use Only

Controls Included

Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
The following compounds were tested for cross-reactivity with the Direct Estradiol ELISA kit with estradiol cross-reacting at 100%.
Estradiol 100%
Estriol 1.6%
Estrone 1.3%
Progesterone 0.1%
Cortisol 0.1%
This assay should not be used for patients being treated with the drug fulvestrant (Faslodex®) which cross reacts with estradiol and could lead to a falsely elevated test result.

Assay Principle

The principle of the Estradiol ELISA Assay Kit enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled estradiol (present in standards, controls and patient samples) and an enzyme-labelled estradiol (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of unlabeled estradiol in the sample. A set of standards is used to plot a standard curve from which the amount of estradiol in patient samples and controls can be directly read.

Related Products

Estrone ELISA Assay Kit
Total Estrogens ELISA Assay Kit
Estrone ELISA Assay Kit

Additional Information

Assay Background

Estradiol is one of the main components of naturally occurring estrogens and is the major estrogen secreted during the menstrual cycle. The serum levels of estradiol are low during the follicular phase rising gradually until about one day before ovulation when a marked rise in the estradiol level occurs (Ovulatory Peak). The estradiol level falls rapidly at, or right after ovulation and is again within the levels of the follicular phase. There is a second rise of estradiol around day 21 of the cycle (Luteal Peak). The levels then decline gradually to the lowest level at the onset of the next menstrual cycle.

Typical Standard Curve

Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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