Estradiol ELISA Assay Kit


The Eagle Biosciences Estradiol ELISA Assay Kit is intended for the quantitative determination of Estradiol in human serum. The Estradiol ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: ESD31-K01 Categories: , ,

Estradiol ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 10 pg/mL
Dynamic Range: 20–3200 pg/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 µL

Controls Included

Additional Information

Assay Background

Estradiol is one of the main components of naturally occurring estrogens and is the major estrogen secreted during the menstrual cycle. The serum levels of estradiol are low during the follicular phase rising gradually until about one day before ovulation when a marked rise in the estradiol level occurs (Ovulatory Peak). The estradiol level falls rapidly at, or right after ovulation and is again within the levels of the follicular phase. There is a second rise of estradiol around day 21 of the cycle (Luteal Peak). The levels then decline gradually to the lowest level at the onset of the next menstrual cycle.

Assay Principle

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled estradiol (present in standards, controls and patient samples) and an enzyme-labelled estradiol (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of unlabeled estradiol in the sample. A set of standards is used to plot a standard curve from which the amount of estradiol in patient samples and controls can be directly read.

Typical Standard Curve


Product Manual