Total PSA ELISA Assay


The Total PSA ELISA Assay (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of Total Prostate Specific Antigen in human serum. The Total Prostate Specific Antigen (PSA) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: PSA31-K01 Categories: , ,

Total PSA ELISA Assay

The Total PSA ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.5–100 ng/mL
Incubation Time: 105 minutes
Sample Type: Serum
Sample Size: 25 μL
Alternative Names: Total Prostate Specific Antigen ELISA, Total Human PSA ELISA, Total Prostatic Specific Antigen ELISA
Controls Included

Assay Principle

The principle of the following enzyme immunoassay test follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for total PSA is immobilized onto the microplate and another monoclonal antibody specific for a different region of PSA is conjugated to horse radish peroxidase (HRP). Total PSA from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of total PSA in the sample. A set of standards is used to plot a standard curve from which the amount of total PSA in patient samples and controls can be directly read.

Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.

Related Products

Total Prostatic Specific Antigen (tPSA) LIA Assay Kit
Free Prostate Specific Antigen (fPSA) ELISA
Total Estrogens ELISA Assay Kit

Additional Information

Assay Background

Prostate specific antigen (PSA) is a 33-kDa glycoprotein secreted by epithelial cells of the prostate gland. In human serum, PSA is primarily complexed with α1-antichymotrypsin, and to a lesser extent with other serum proteins. Only a small portion of PSA is present as the free form (free PSA). The expected normal level of total PSA in male serum is lower than 4 ng/mL. A rise in the concentration of PSA indicates prostate pathology, including benign prostatic hyperplasia and prostate cancer.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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