Progesterone ELISA Assay Kit


The Eagle Biosciences Progesterone ELISA Assay Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Progesterone concentration in human serum or plasma. This Progesterone ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM006 Categories: , ,

Progesterone ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.05 ng/mL
Dynamic Range: 0.2 – 40 ng/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 20 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Progesterone is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone is the major naturally occurring human progestagen. Progesterone is important for aldosterone (mineralocorticoid) synthesis, as 17-hydroxyprogesterone is for cortisol (glucocorticoid). Progesterone levels are relatively low in children and postmenopausal women. Adult males have levels similar to those in women during the follicular phase of the menstrual cycle. In women, progesterone levels are relatively low during the preovulatory phase of the menstrual cycle, rise after ovulation, and are elevated during the luteal phase. If pregnancy occurs, progesterone levels are maintained at luteal levels initially. After delivery of the placenta and during lactation, progesterone levels are very low. The fall in progesterone levels following delivery is one of the triggers for milk production .

Progesterone is produced in the adrenal glands, the gonads (specifically after ovulation in the corpus luteum), the brain, and, during pregnancy, in the placenta. Progesterone converts the endometrium to its secretory stage to prepare the uterus for implantation. If pregnancy does not occur, progesterone levels will decrease, leading, in the human, to menstruation. Progesterone belongs to the group of neurosteroids that are found in high concentrations in certain areas in the brain and are synthesized there. Neurosteroids affect synaptic functioning, are neuroprotective, and affect myelinization. Progesterone has multiple effects outside of the reproductive system. Progesterone is thermogenic, it reduces spasm and relaxes smooth muscle. Bronchi are widened and mucus regulated. Progesterone acts as an antiinflammatory agent and regulates the immune response. Progesterone also assists in thyroid function, in bone building by osteoblasts.

Assay Principle

In the Progesterone ELISA Assay Kit, the (antigen) in the sample competes with the antigenic Progesterone conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Progesterone coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution (H2SO4) is added. The colour intensity is inversely proportional to the Progesterone concentration of in the sample. Progesterone concentration in the sample is calculated through a calibration curve.


Product Manual


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Recent Citations:

Alexander, L.The Impact of high protein intake on body composition in active females during a hypoenergetic diet (Dissertation, University of Stirling, 2015).