Progesterone ELISA Assay Kit

$190.00

The Eagle Biosciences Progesterone ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the direct quantitative determination of progesterone in human serum. The Progesterone ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: PRG31-K01 Categories: , ,

Progesterone ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.3–60 ng/mL
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 25 μL

Controls Included

Additional Information

Assay Background


Progesterone is a C-21 female sex steroid hormone with a variety of physiological effects. In the follicular phase of the menstrual cycle, progesterone is produced in low levels. It increases to the LH peak and then sharply rises 3 to 4 days later to higher levels, remaining elevated through the 10th to 12th days after the LH peak. Then there is a sharp decline to the low levels of the follicular phase. It is responsible for the induction of the cyclic changes in the endometrium of the uterus allowing implantation and successful growth of the fertilized ovum and maintenance of pregnancy. Progesterone measurements are used in documenting ovulation and in the management of difficulties during the first trimester of pregnancy. Levels of progesterone may be useful in the evaluation of sterility due to luteal phase defects, prediction of impending abortion, and the diagnosis of ectopic pregnancy. Normal values of progesterone may be affected by drugs such as, oral contraceptives, superovulatory drugs, estrogen replacement therapy medication, and GnRH analogues. The removal of ovarian function following surgical oophorectomy or chemotherapy may influence serum progesterone values.

Assay Principle


The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of progesterone in the sample. A set of standards is used to plot a standard curve from which the amount of progesterone in patient samples and controls can be directly read.

Typical Standard Curve


Manual

Product Manual