Progesterone ELISA Assay Kit
The Progesterone ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.3–60 ng/mL
Incubation Time: 80 minutes
Sample Type: Serum
Sample Size: 25 μL
Progesterone is a C-21 female sex steroid hormone with a variety of physiological effects. In the follicular phase of the menstrual cycle, progesterone is produced in low levels. It increases to the LH peak and then sharply rises 3 to 4 days later to higher levels, remaining elevated through the 10th to 12th days after the LH peak. Then there is a sharp decline to the low levels of the follicular phase. It is responsible for the induction of the cyclic changes in the endometrium of the uterus allowing implantation and successful growth of the fertilized ovum and maintenance of pregnancy. Progesterone measurements are used in documenting ovulation and in the management of difficulties during the first trimester of pregnancy. Levels of progesterone may be useful in the evaluation of sterility due to luteal phase defects, prediction of impending abortion, and the diagnosis of ectopic pregnancy. Normal values of progesterone may be affected by drugs such as, oral contraceptives, superovulatory drugs, estrogen replacement therapy medication, and GnRH analogues. The removal of ovarian function following surgical oophorectomy or chemotherapy may influence serum progesterone values.
SPECIMEN COLLECTION AND STORAGE
Approximately 0.1 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.