Estrone ELISA Assay Kit

$250.00

The Estrone ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Estrone in human serum. The Estrone ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: ESN31-K01 Categories: , ,

Estrone ELISA Assay Kit

The Estrone ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 3 pg/mL
Dynamic Range: 10–2000 pg/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 µL
Controls Included


Specimen Collection and Storage for Estrone ELISA
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
Specificity
The following compounds were tested for cross-reactivity with the Direct Estrone ELISA kit with estrone cross-reacting at 100%:
Estrone 100%
Estrone-3-Sulfate 4.9%
17B-Estradiol 2.2%
Estrone-3-Glucuronide 1.2%
17B-Estradiol-3-Glucuronide 0.14%
The following steroids were tested but cross-reacted at less than 0.1%: Androstenedione, Cholesterol, Corticosterone, Cortisol, Cortisone, DHEAS, Diethylstilbesterone, Estriol, 17βEstradiol-3-Glucuronide, Estradiol-Sulfate, Progesterone, 17-OH Progesterone and Testosterone.


Assay Principle

The principle of the Estrone ELISA Assay Kit enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of estrone in the sample. A set of standards is used to plot a standard curve from which the amount of estrone in patient samples and controls can be directly read.


Related Products

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Total Estrogens ELISA Assay Kit

Additional Information

Assay Background


Estrone is a steroid like estriol and estradiol, belonging to the class of estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the estrone level shows a slight increase. The production of estrone then increases markedly to peak at around day 13. The peak is of short duration and by day 16 of the cycle levels will be low. A second peak occurs at around day 21 of the cycle and if fertilization does not occur, then the production of estrone decreases.

Typical Standard Curve


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