Testosterone ELISA Assay Kit

$190.00

The Testosterone ELISA Assay Kit is intended for the direct quantitative determination of Testosterone in human serum. The Testosterone ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: TST31-K01 Categories: , ,

Testosterone ELISA Assay Kit

Testosterone ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.022 ng/mL
Dynamic Range: 0.08–16.7 ng/mL
Incubation Time: 75 minutes
Sample Type: Serum
Sample Size: 50 µL
Controls Included


Assay Principle for Testosterone ELISA Assay Kit


The principle of the Testosterone ELISA test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and (patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed is inversely proportional to the concentration of testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.


SPECIMEN COLLECTION AND STORAGE for Testosterone Assay
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
POTENTIAL BIOHAZARDOUS MATERIAL
Human serum that may be used in the preparation of the standards and controls has been tested and found to be nonreactive for Hepatitis B surface antigen and has also been tested for the presence of antibodies to HCV and Human Immunodeficiency Virus (HIV) and found to be negative. No test method however, can offer complete assurance that HIV, HCV and Hepatitis B virus or any infectious agents are absent. The reagents should be considered a potential biohazard and handled with the same precautions as applied to any blood specimen.


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Additional Information

Assay Background


Testosterone is the most important male sex hormone, it is responsible for genital development, beard growth, muscle development and general male characteristics. The measurement of serum or plasma levels is an index of leydig cell function and high or low values correlate well with hypo- or hypergonadism. In females small amounts of testosterone are produced by the adrenals and ovaries. High levels of testosterone in females indicates excessive androgen production and are found in progressive hirsutism and virilization, Cushing’s syndrome and a deficiency in one or more of the specific enzymes required for normal steroid biosynthesis.

Typical Standard Curve


Documents


Publications

Citations


Cardaci, T.D., Machek, S.B., Wilburn, D.T., et al. High-Load Resistance Exercise Augments Androgen Receptor-DNA Binding and Wnt/b-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content. Nutrients (2020) https://doi.org/10.3390/nu12123829