Mouse Monoclonal Anti-MLH1 Antibody Clone PBM-5A6

Mouse Monoclonal Anti-MLH1 Antibody Clone PBM-5A6

The Mouse Monoclonal Anti-MLH1 Antibody Clone PBM-5A6 is For Research Use Only

Immunogen: Full Size Recombinant Protein
Alternative Names: MutL Homolog 1
Host: Mouse
Class: IgG1, Kappa
Specificity: Human
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Colon Tissue
Demasking: Temperature, Tris buffer, pH 9 (12-310003)
Staining: Nuclear
Reagent provided: This antibody is immunoglobulin IgG1 diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
Usage dilution:
10-310039-7: Ready to Use
10-310039-01; 10-310039-05; 10-310039-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Tris Buffer, pH 9 (REF 12-310003) or Tris-EDTA buffer, pH 9 (REF 12-310008).
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Storage: Store at 2-8°C and in the dark. Do not use after expiration date.

Background: MLH1 protein is deficient in a high proportion of patients with microsatellite instability (MSI). It has been shown that immunohistochemical analysis of MLH1 expression is a trustworthy and reliable method for the routine detection of most cases of colorectal carcinomas with high MSI frequency, which is often found in hereditary non-polypous colorectal cancer (HNPCC) and in 15% of cases of sporadic colorectal cancer. It is suggested that tumors negative for MLH1 may be associated with greater patient survival without relapse.

Product Developed and Manufactured by Diagomix.