Mouse Monoclonal Anti-AMACR Antibody Clone PBM-G8
The Mouse Monoclonal Anti-AMACR Antibody Clone PBM-G8 is For Research Use Only
Alternative Names: Alpha-methylacyl-CoA racemase
Class: IgG2a, kappa
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Prostate adenocarcinoma
Demasking: Temperature, Tris buffer, pH 9 (12-310003)
Reagent provided: This antibody is immunoglobulin IgG2a, kappa diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and and 0.05% ProClin™ 300.
10-310010-7: Ready to Use
10-310010-01; 10-310010-05; 10-310010-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Tris buffer, pH 9 (12-310003).
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Storage: Store at 2-8°C and in the dark. Do not use after expiration date.
Background: AMACR (alpha-methyl acyl-coenzyme A racemase A) is expressed in cells of precancerous intraepithelial neoplasia of the prostate (PIN) with high malignant potential and prostate adenocarcinoma, but is present at low or undetectable levels in normal glandular epithelial cells of the prostate and benign hyperplasia cells. AMACR is the preferred marker for prostate cancer. In addition, AMACR is expressed in normal epithelium and in cells of other adenocarcinoma species.
Product Developed and Manufactured by Diagomix.