Certolizumab (CIMZIA) ELISA Assay Kit

$1,465.00

The Certolizumab (CIMZIA) ELISA Assay Kit is intended for the quantitative analysis of free certolizumab in serum and plasma samples. The Certolizumab (CIMZIA) ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: CER-FD-CIM Categories: , ,

Certolizumab (CIMZIA) ELISA Assay Kit

The Certolizumab (CIMZIA) ELISA Assay Kit is For Research Use Only

Size: 12 x 8 wells
Sensitivity: 20 ng/mL
Standard Range: 0 – 1000 ng/mL
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Name: Cimzia

Controls Included


Assay Background

Certolizumab pegol is a pegylated monoclonal antibody against the tumour necrosis factor- alpha (TNF-alpha). It is formed with a humanized Fab fragment of 50 kDa, from an lgG l isotype, fused to a 40 kDa polyethylene glycol moiety replacing the Fe antibody region. The absence of the Fe region was ideated to prevent complement fixation and antibody- mediated cytotoxicity as well as to markedly increase its half-life. Certolizumab does not require glycosylation for active function and hence, its production is significantly more affordable when compared to other existing TNF-alpha therapies as it can be done directly in bacterial hosts such as E.coli.

Assay Principle

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for certolizumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to certolizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of certolizumab in the sample or standard. Results of samples can be determined directly using the standard curve.


Related Products

Anti-Certolizumab (CIMZIA) Quantitative ELISA Assay Kit
Certolizumab (CIMZIA) Neutralizing Antibody ELISA Assay Kit

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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