Mouse Monoclonal Anti-EpCAM Antibody Clone VU-1D9
The Mouse Monoclonal Anti-EpCAM Antibody Clone VU-1D9 is For Research Use Only
Immunogen: Full Size Recombinant Protein
Alternative Names: Epithelial Cell Adhesion Molecule
Class: IgG1, Kappa
Application: IHC on FFPE (formalin fixed paraffin embedded tissue sections)
Positive Control: Colon Tissue, Thyroid Gland
Demasking: Enzymatic, Proteinase K
Staining: Membranous and Cytoplasmic
Reagent provided: This antibody is purified immunoglobulin IgG1 diluted in 10 mM Phosphate buffered saline (PBS), pH 7.4 containing 1% bovine serum albumin (BSA) and 0.05% ProClin™ 300 as antimicrobial agent.
10-310017-7: Ready to Use
10-310017-02; 10-310017-05; 10-310017-1: Dilute 1:50 to 1:100 with Antibody Diluent (REF 12-310001) before use. Optimum dilution factor may vary depending on the specimen and preparation process and should be determined by each individual investigator.
Epitope retrieval: Staining of formalin fixed, paraffin embedded tissue sections is significantly enhanced by pretreatment with Proteinase K.
Staining procedure: Incubate this antibody with tissue section for 30-60 minutes at room temperature. Follow the instructions from the selected detection system.
Storage: Store at 2-8°C. Do not use after expiration date.
Background: EpCAM (epithelial cell adhesion molecule, surface glycoprotein) is expressed in normal epithelial cells and in carcinomas. These antibodies are used (as part of antibody panels) for the differential diagnosis of adenocarcinoma and mesothelioma, basal cell carcinoma and squamous skin cancer. Antibodies can also be used to detect micrometastases in the lymph nodes in patients with esophageal carcinoma.
Product Developed and Manufactured by Diagomix.