iLite GM-CSF Assay Ready Cells

$1,240.00

The iLite GM-CSF Assay Ready Cells can be used for the quantification GM-CSF activity, GM-CSF inhibitor activity, and for determination of neutralizing antibodies against such either in buffer systems or human serum. The iLite GM-CSF Assay Ready Cells are for research use only.

SKU: BM4050 Categories: ,

iLite GM-CSF Assay Ready Cells

iLite GM-CSF Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite GM-CSF ARCs are intended for:

  • Quantification of GM-CSF activityy
  • Determination of GM-CSF neutralizing activity

Content: >250 µL of iLite® Assay Ready Cells suspended in RPMI 1640 with 20% FBS, mixed 1:1 with cryoprotective medium from Lonza
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only


Key benefits of iLite® Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (>10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Description

iLite® GM-CSF Assay Ready Cells are a genetically engineered reporter gene cell line responsive to granulocytemacrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.


Products Related to iLite GM-CSF Assay Ready Cells

TNF-alpha Assay Ready Cells
iLite IL-23 Assay Ready Cells
iLite IL-12 Assay Ready Cells

Additional Information

Background


Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine which stimulates the production of granulocytes and monocytes from bone marrow precursors (1). As such, it serves as an important key in both humoral and cell mediated immunity. Recombinant GM-CSF has several therapeutic uses; in order to accelerate leukocyte recovery after bone marrow transplantation, to replenish leukocytes after chemotherapy and for treatment of fungal infections. The immunostimulatory effects of GM-CSF have also been used in oncolytic viruses modified to include genes coding for GM-CSF, thus enhancing recruitment of the immune response to tumor cells. In addition, the discovery of a pro-inflammatory role of GM-CSF in autoimmune disease has led to development of several GM-CSF inhibitor drugs (2, 3)

Manual

Product Manual


Frequently Asked Questions


Publications

References


1. Burgess AW, Camakaris J, Metcalf D. Purification and properties of colony-stimulating factor from mouse lung-conditioned medium. Journal of Biological Chemistry 252(6):1998-2003 (1977).
2. Hamilton, JA. Colony-stimulating factors in inflammation and autoimmunity. Nature Reviews Immunology 8(7):533-44 (2008).
3. Kim JH, Oh JY, Park BH, Lee DE, Kim JS, Park HE, Roh MS, Je JE, Yoon JH, Thorne SH, Kirn D, Hwang TH. Systemic armed oncolytic and immunologic therapy for cancer with JX-594, a targeted poxvirus expressing GM-CSF. Molecular Therapy 14(3):361-70 (2006).