iLite G-CSF Assay Ready Cells


The iLite® G-CSF Assay Ready Cells can be used for the quantification G-CSF activity and G-CSF inhibitor activity, either in buffer systems or human serum. The Eagle Biosciences iLite® G-CSF Assay Ready Cells are for research use only.

SKU: BM4055 Categories: ,

iLite G-CSF Assay Ready Cells

iLite G-CSF Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite G-CSF ARCs are intended for:

    • Quantification of functional G-CSF
    • Quantification of G-CSF inhibitor activity

Content: 250 µL of Assay Ready Cells suspended in cryoprotective medium from Amsbio
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only

Key benefits of iLite Assays

  • Highly specific reporter gene cell lines
  • Very sensitive cell line responses (10 fold inductions)
  • Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
  • Assays within a workday (typically 4-7 hour assays)
  • Normalization gene, which eliminates unwanted matrix effects

Product Description

iLite G-CSF Assay Ready Cells are avian DT-40 cells which have been genetically engineered and optimized to be responsive to G-CSF, resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.

Products Related to iLite G-CSF Assay Ready Cells

iLite GM-CSF Assay Ready Cells
Human G-CSF ELISA Assay Kit
iLite VEGF Assay Ready Cells

Additional Information


Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates cells in the bone marrow to differentiate into granulocytes which are subsequently release into the blood. The G-CSF-Receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates proliferation and differentiation into mature blood cells. Different types of pharmaceutical analogs are used as a therapeutic agent to stimulate the bone marrow to produce more neutrophils (white blood cells) to fight infection in patients undergoing chemotherapy for cancer (1,2).

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Frequently Asked Questions



1. Deotare U, Al-Dawsari G, Couban S, Lipton JH (September 2015). “G-CSF-primed bone marrow as a source of stem cells for allografting: revisiting the concept”. Bone Marrow Transplantation. 50 (9): 1150–6.
2. Tay J, Levesque JP, Winkler IG (December 2016). “Cellular players of hematopoietic stem cell mobilization in the bone marrow niche”. International Journal of Hematology. 105: 129–140.

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